Research On Effects Of Tea Polyphenols On Skin Wound Healing In Mice

Tea polyphenols,the main functional component of tea,showed a potential efficacy and broad market application prospect of wound healing.This thesis adopts animal experiments to observe and analyze the whole process of mouse wound healing and the characteristics and duration of each phase.By innovatively adopting a phased and hierarchical experimental design,this thesis explored the effect of 0.5%tea polyphenols on mouse skin wound healing and compared it with some commercially available drugs.The main research contents and results of this thesis are as follows:(1)The physicochemical composition of tea polyphenols was determined by HPLC,and its antibacterial effect was verified.It was clearly established that the 0.5%tea polyphenols have good wound disinfection effect which was similar to PVP-I.(2)The overall macroscopic effects of each drug on wound healing were investigated by histomorphological observation of mouse skin wounds by visual observation,Masson trichrome staining,and Sirius red staining.The results showed that 0.5%tea polyphenol could shorten the time of wound closure,and its effect was similar to rhEGF;and this concentration of tea polyphenols could regulate the ratio of collagen in the new tissue of mouse wounds and reduce the possibility of scarring after wound healing.(3)ELISA and IHC were used to determine and observe the dynamic changes of inflammatory factors in mice to investigate the effect of tea polyphenols on the inflammatory response phase.It was found that 24 h after wound production,tea polyphenols significantly reduced the concentration of some inflammatory factors in the serum and the number of inflammatory cells at the wound sites in mice,which had a better anti-inflammatory effect.(4)IHC,RT-qPCR and Western blot were used to determine the dynamic changes of growth factor levels in mouse wound tissues to investigate the effects of tea polyphenols on the new tissue formation stage.The results showed that tea polyphenols had the effect of promoting the expression and concentration of vascular endothelial growth factor(VEGF)and capillary regeneration at the wound sites in mice,which was beneficial to the formation of new tissues.In conclusion,0.5%tea polyphenols are helpful to the skin wound healing in mice and has a good basis and prospect for further development.The results provide a theoretical basis for the development of tea polyphenols as a cost-effective wound treatment drug and help to broaden the utilization channels and enhance the utilization value of tea leaves,and also respond to the call of the national agricultural supply-side structural reform.