Separation And Identification Of Chemical Components In Nelumbinis Plumula

Coronary heart disease has a high morbidity and mortality,and the age of the diseased population tends to be younger.Coronary heart disease also has a genetic predisposition and has become one of the diseases that seriously threaten human health in the world.The main cause of high mortality from coronary heart disease is acute myocardial infarction(AMI).The current methods of treating myocardial infarction mainly include drug therapy,interventional therapy and bypass surgery.The most commonly used interventional therapy is reperfusion therapy,but this method may cause ischemia-reperfusion injury during the treatment process,which will further aggravate the situation.At present,there is a lack of specific drugs to improve ischemia-reperfusion injury.In order to improve the survival rate of patients with acute myocardial infarction,it is particularly important to find new drugs to improve myocardial ischemia-reperfusion injury.Based on the relevant literature reported in the past 30 years,it is found that only about 60 compounds have been isolated and identified from the Nelumbinis Plumula,including: alkaloids,flavonoids and their glycosides,polysaccharides,volatile oils and sterols and other chemical components.Alkaloids,flavonoids and their glycosides are the main components in the Nelumbinis Plumula.It has been reported in the literature that Nelumbinis Plumula is clinically used to treat coronary heart disease.Firstly,this study using HL-1 cells and HL-1-STAT3-Luc cell lines to construct a model of ischemia-hypoxic reoxygenation myocardial injury,and evaluates the activity of Nelumbinis Plumula 80% Ethanol extracts are extracted and separated by column chromatography on silica gel.Screen out the protective effect on HL-1 cells under hypoxia/reoxygenation conditions,as well as the part that promotes the expression of STAT3 in the HL-1-STAT3-luc cell line.Then use TLC-EFISI-MS technology for preliminary structural characterization of the activity part.In addition,in order to verify the accuracy of the structures characterized by TLC-EFISI-MS,a systematic ally chemical composition study was carried out on the active Fractions in the Nelumbinis Plumula that promote the expression of STAT3.1.TLC-EFISI-MS rapid characterization of the Structure of the chemical components in the active part of Nelumbinis PlumulaA model of ischemia-hypoxia/reoxygenation myocardial injury was constructed by HL-1 cells and HL-1-STAT3-Luc cell lines.Use this model to evaluate the protective effect of different extracts of Nelumbinis Plumula on HL-1 cells and the ability to promote the expression of STAT3 in the HL-1-STAT3-Luc cell line.Screen out the dichloromethane extraction Fraction with the best effect.Therefore,the dichloromethane extraction layer is mainly studied.Use TLC-EFISI-MS technology to preliminary structural characterization of the active Fr.1 and Fr.3 parts,by the mass spectrometry analysis: γ-tocopherol(spot 1),loliolide or isololiolide(spot 3),(+)-Phaseic acid or(-)-Phaseic acid(spot 4),soya-cerebroside I(spot 6).It was discovered for the first time that the heart of lotus seeds may also contain tocopherol,sphingolipids and terpenoids.2.Isolation and identification of the chemical components in the Nelumbinis Plumula with myocardial protective effectIn order to verify the accuracy of the structure characterized by TLC-EFISI-MS,and to provide a material basis for screening active ingredients with myocardial protection,a systematic chemical composition study was carried out on activity part.46 compounds were separated,purified and identified,including 7 alkaloids(1-7),7flavonoids(8-14),11 terpenes(15-25),3 tocopherols(26-28),2 phenylpropanoids(29-30),4 sterols(31-34),3 sphingolipids(35-37),2 phenol glycosides(38-39),1coumarin(40),5 simple aromatic compounds(41-45)and 1 nucleoside(46).According to the results of the above-mentioned research on the chemical components of Fr.1 and Fr.3,the four components identified on TLC via TLC-EFISI-MS were confirmed asγ-tocopherol,isololiolide and(+)-Phaseic acid,soya-cerebroside I.The activity of tocopherols(27-28),terpenoids(15-24)and new compounds(1),It was found that compounds 16,18,19 and 23 had significantly protective effects on mouse cardiomyocytes(HL-1)under hypoxia(P < 0.001);and compounds 15,17,20,22 and24 have moderate protective effect on mouse cardiomyocytes(HL-1)under hypoxia(P< 0.01).In addition,compounds 15,16,19,20 and 22 can significantly promote HL-1-STAT3-Luc The expression of STAT3 in cell lines.