| Purpose:The purpose of this paper is to detect the diversity and differences in intestinal flora structure between patients with stable angina pectoris with spleen deficiency and phlegm turbidity syndrome and normal people by 16 S r DNA high-throughput sequencing technology,and to reveal the intrinsic biology of coronary heart disease stable angina pectoris with spleen deficiency and phlegm turbidity syndrome,and to provide reference for the study of early screening,early diagnosis and prevention and treatment of patients with stable angina pectoris of coronary heart disease with spleen deficiency and phlegm turbidity syndrome.Methods:From October 2021 to December 2021,60 patients with stable angina pectoris with the syndrome of spleen deficiency and phlegm turbidity who were treated in the Affiliated Hospital of Liaoning University of Traditional Chinese Medicine were collected as the observation(GXBB)group,and 60 healthy people were recruited during the same period.Control(ZC)group.The clinical data and feces of subjects in each group were collected,and16 S r DNA high-throughput sequencing technology was used to compare the differences in the abundance,structure and species diversity of intestinal flora in the fecal samples of each group.Results:1.Baseline data: There was no statistical difference in gender between the case group and the normal group,but there were differences in age,hypertension,diabetes,and dyslipidemia.2.Intestinal flora results:(1)A total of 9,002,528 Raw tags and 7,469,056 Valid Tags were obtained from the two groups of samples in this sequencing,including 3,588,157 in the case group and 3,880,899 in the normal group;on average,each sample produced 62,242 Valid Tags,The effective sequence length is concentrated between 400-500 bp,the average proportion of effective sequences is 84.29%,the average proportion of Q20 is 98.17%,the average proportion of Q30 is 94.38%,and the average content of GC is 53.16%;(2)Alpha diversity analysis: Chao1 index(P=0.00<0.05),Observed species index(P=0.00<0.05),with statistical significance,suggesting that the two groups of samples have differences in bacterial abundance;Shannon index(P=0.48 > 0.05),Simpson index(P=0.48 > 0.05)=0.59>0.05),Goods coverage index(P=0.05),and Pielou-e index(P=0.38>0.05),there was no statistical significance,suggesting that there were no differences in diversity,bacterial coverage and evenness between the two groups of samples.(3)Beta diversity analysis: The two groups of samples showed a certain aggregation,and some samples were separated,indicating that the two groups of samples had both certain similarities and certain differences in the structure of intestinal flora.(4)The dilution curve shows that the sequencing depth is sufficient,covering almost all species.(5)Comparison of results at different taxonomic levels:1)At the phylum level,the observation group compared to the control group: the abundance of Actinobacteria increased,and the abundance of Bacteroidota and Verrucomicrobia decreased,and the difference was statistically significant(P< 0.05);2)At the class level,the observation group compared to the control group: the abundance of Coriobacteriia and Verrucomicrobiae increased,and the abundance of Bacteroidia and Firmicutes decreased,and the difference was statistically significant(P< 0.05);3)At the order level,the observation group compared to the control group: Coriobacteriales,Verrucomicrobiales,Clostridia UCG-014,Christensenellales,and Peptostreptococcales-Tissierellales increased in abundance;The abundance of Bacteroidales,Lactobacillales,Acidaminococcales and Burkholderiales decreased,and the difference was statistically significant(P<0.05);4)At the family level,the observation group compared to the control group: Akkermansiaceae,Clostridia UCG-014 unclassified,Coriobacteriaceae,Christensenellaceae,Peptostreptococcaceae increased in abundance;Lachnospiraceae,Bacteroidaceae,Acidaminococcaeceae,Tannerellaceae,Sutterellaceae,Butyricicoccaceae and Marinifilaceae decreased,and the difference was statistically significant(P<0.05);5)At the genus level,the observation group compared to the control group: Dialister,Akkermansia,Clostridia＿UCG-014＿unclassified,Collinsella,Alloprevotella,Mitsuokella,Prevotella 7,CAG-352,Ligilactobacillus,Limosilactobacillus,Lactobacillus,CAG-873 increased;Bacteroides,Phascolarctobacterium,Lachnospira,Parabacteroides,Lachnoclostridium,Eubacterium eligens group,Parasutterella,Sutterella,Lachnospiraceae UCG-010,Bilophila,Butyricicoccus,Incertae Sedis,Flavonifractor,Lachnospiraceae ND3007 group,Lachnospiraceae UCG 004,Butyricimonas,Colidextribacter,Odoribacter decreased,and the difference was statistically significant(P<0.05).Conclusions:1.Comparing the intestinal flora of patients with spleen deficiency and phlegm turbidity syndrome with stable angina pectoris,there are significant differences in the level of phylum,class,order,family,and genus in the intestinal flora,which is specifically reflected in the abundance of flora.2.The reduction of bacteria such as Bacteroides,Parabacteroides,Lachnospira,Lachnospiraceae UCG-010,Phascolarctobacterium,Sutterella,Eubacterium eligens group,Parasutterella,and the increase of bacteria such as Collinsella,Prevotella7,Mitsuokella and Dialister may be involved in stable angina pectoris Atherosclerosis process in patients with spleen deficiency and phlegm syndrome.3.The role of intestinal flora in the formation of atherosclerosis in patients with stable angina pectoris with spleen deficiency and phlegm-dampness syndrome may be attributed to metabolic abnormalities caused by changes in flora abundance and regulation of inflammation and immunity,but the detailed mechanism remains to be seen further research. |
PDF Full Text Request