Metabonomic Study On The Animal Model And Clinical Patients Of Kidney Yang Deficicency

"kidney yang deficicency"is the one of the basic morbid situation in Chinese medicine, which usually existing in the onset and progression of many different diseases, such as chronic heart failure and renal failure. Metabolites can reflect the actual information of physiological and pathological status. With the development of modern chemical instrumental analysis and chemometrics analysis, metabonomics has been applied in lots of fields including morbid/disease diagnosis, toxicologic evaluation and so on. Therefore, we presume there might be specific changes of metabolic profiling in"kidney yang deficicency"and metabonomics method can find the changes.In the article, we took different kinds of urine samples from patients and animals as the subjects to explain the variations of metabolites with"kidney yang deficicency"in the version of"metabonomics".We copyed the"kidney yang deficicency"animal model using ?hydrocortisone and adenine and urine samples of defferent timepoints were obtained. Subsequently, derivatized samples were infused in GC-MS in conjunction with modern multivariate statistical techniques to identify significant biochemical changes in different kinds of samples. The metabonomics approach enables us to visualize significant alterations in metabolite expression patterns as a result of hydrocortisone and adenine induced"kidney yang deficicency"rats metabolic responses. Alanine and tyrosine showed the same various trend in two kinds chemical regent induced"kidney yang deficicency"rats.Clinical urine samples of"kidney yang deficicency"of chronic heart failure and"kidney yang deficicency"of renal failure were also used to process metabonomics study. The results showed that the concentration of olic acid and tyrosine had significiant change in the urine of"kidney yang deficicency"patients.As a results, tyrosine concentration has significantly increased in both"kidney yang deficicency"rats and patients. The concentration of olic acid and tyrosine has the same various trend in adenine induced"kidney yang deficicency"rats and"kidney yang deficicency"patients of chronic heart failure and renal failure. Therefore, we may primarily concluded that the change of tyrosine concentration is a potential biomarker of"kidney yang deficicency"syndrom.