Research Advancement Of Gynecological Blood Stagnation And The Experimental Research Of Effect Which Blood Activating And Stasis Removing Leads To The Blood Stasis Rat-Model

Objective: Sup-chapter intends to summarize and approach the forming mechanism and the therapeutical principle and method of gynecological blood stagnation, as well as the blood activating and stasis removing mechanism, through extensive and penetrating researches on pertinent literature about clinical and empirical study advancement of common gynaecopathia blood stagnation. And it is also for providing theoretical foundation for the animal experiment.Midst —chapter aims at further approach of the forming mechanism and the blood activating and stasis removing mechanism by observing the changes of E₂, P, E₂R, PR, Ca²⁺, hemorrheology, utero-ovarian histomorphology on blood stasis rat-model, and also by observing the effect on the changes of the above-mentioned factor on blood stasis rat—model which is done by the method of blood activating and stasis removing.Methods : Sup-chapter extensively refers to pertinent literature of clinical and empirical study advancement of common gynaecopathia blood stagnation, then thoroughly researches and summarizes are done.Midst-chapter is about animal experimental research: forty SD rats were divided into four groups , that is blank control group(A), blood stasis model group(B),mode1+ Yunnan white powder group(C), model+ GongXueTing group(D), each group 10 rats. Each group was intragastric administered by turns aequales physic liquor: isotonic Na chloride, Yunnan white powder suspension, GongXueTing apozem. When the last administration was finished, the last-three groups were made blood stasis model. 20 hours later, took blood , uterine and ovary, and carried out the observation of hemorrheology, blood-serum E₂, P level, E₂Rn PR of uterine and ovary, blood-serum Ca²⁺ content, HE staining of uterine and ovary section. Experimental results were made interclass contrast with T-test and ANOVA to compare the significance of data differentiation.Results: Sup-chapter literature research discovers that : 1, The formof gynecological blood stagnation is mainly due to the following aspects: (1)The changes of hemorheology and hemodynamics. (2)The changes of local microcirculation and histomorphology. (3)The changes of endocrine system hormone level. (4) The disorder of immune functional status in corpore. (5)The changes of content of microelement (especially Zn, Cu). 2, The blood activating and stasis removing mechanism is chiefly related to the following aspects: (1)Estrogen effectiveness. (2)Improving the main index of hemorheology, and improving uterine microcirculation. (3)djusting the blood clotting system and the fiber dissolving function. (4)Protecting vascular endothelial cell and its function. (5)Obviously inhibiting the contraction of uterine smooth muscle. (6)Analgesic effectiveness. (7) Anti-inflammatory action. (8)Enhancing immune function(9)Sedation 3, The therapeutic methods for gynecological blood stagnation: ?The therapy of differentiation of symptoms and signs is the most commonly used and also the most basic method: the method of warming the meridians and promoting blood flow ,as well as the method of promoting qi to activate blood , are firstly chosen in sthenia syndrome; in contrast the method of invigorating the kidney and promoting blood flow is firstly chosen in asthenia syndrome. In addition, the method of removing blood stasis and homeostasis is emphasized in hemorrhagic disease due to blood stasis. ?Other therapeutic methods , like periodicity treatment for EMS, comprehensive therapy for Chronic Pelvic Inflammatory Disease, or for fallopian tube obstructive infertility, are all have conspicuous effects.Mid-chapter experimental study found that :blood stasis model group compared with blank control group, E2^ Pare degraded obviously (P<0.01) , blood -serum Ca2+value obviously is raised (P<0. 05) .there is no obvious changes to the positive level of E2f^ PR (P >0. 05), however, there is extremely obvious raise in each respect of hemorheology, ovary stroma and proliferative stage endometria shows obvious blood stasis pathological changes, while progestational stage endometria don' t .Comparing the two therapy group to the blood stasis model group, E2 level is prominently raised (P<0. 01) , P value also got the tendency to rise up (P>0. 05) , serum Ca2+ value further prominently step up (P<0. 05) , there is still no obvious difference for the positive ness of E2R> PR (P>0. 05), hemorheological index all got notable degradation (P<0.01), ovary stroma> proliferative stage endometriaN progestational stage endometria are not changed obviously.Conclusion: The forming mechanism of blood stagnation, in addition to hemorheological and histomorphological changes (the detail in the sup-capter literature), probably also lies in: ?Reduction of E2, P level leads to hemorheological abnormality, blood-fat level rise, blood presents sticking, thickness condition. Reduction of E2^ P level leads to the regulating function of blood vessel endothelium factor nitrogen monoxidum(NO), endothelin(ET) likewise reducing. If NO generation reduces, the inhibitory action for ET is weakened, and angiotasis system...