| Isolation and purification technics of Opuntia dillenii Haw. polysaccharide (OPS) , theanti-oxidation effect on OPS-Ia on rat RBC lipid per-oxidative damage, and the effect of OPS-Iaon streptozotocin-induced diabetic mice were studied in this paper. The preliminary research ofpossible mechanisms of antidiabetes of OPS-Ia was done, too. The main results were shown asfollows:1. Isolation and purification of OPSBased on the biologic activity of polysaccharide, the effect of ultrasonic time, ratio materialsto water and pH value on OPS yield were studied by a central composite rotatable second-orderdesign. The results showed that OPS yield was noticeably affected by ultrasonic time, ratio waterto materials and pH in significant order. The pH value was the main factor affecting activity ofOPS, since the activity of OPS extracted in the condition of acid and alkali was very low. Theregression equation could estimate the OPS yield in the range of experiment. According to theregressive equation, the optimum condition of OPS yield was ultrasonic time 46min, ratiomaterials to water 38(mL·g-1) and pH value 7.12.The main three fractions OPS-Ia, OPS-Ib, OPS-Ⅱ' were obtained by DEAE-Sepharose FastFlow and Sephadex glucan column chromatography. Their molecular weight, which isdetermined by gel filtration, is 60kD, 12kD and 167kD respectively.2. The antioxidation effect of OPS-Ia on rat RBC lipid peroxidative damageMethods: The rat RBC was damaged by H2O2 and Fe2+/H2O2 in vitro. The anti-oxidationeffect of the OPS-Ia was researched by observing the changes of hemolysis rate, methemoglobinlevel, erythrocyte membrane LPO content, erythrocyte membrane protein -SH group content,erythrocyte membrane SOD activity, and erythrocyte membrane GSH-Px activity.Result: OPS-Ia could suppress the rat RBC hemolysis rate and the production ofmethemoglobin in rat RBC suspension liquid which was induced by H2O2. Moreover, treatmentof OPS-Ia could significantly inhibit the reduction of erythrocyte membrane protein -SH groupcontent and the increases of LPO content, and increase the SOD activity, GSH-Px activity oferythrocyte membrane which was induced by Fe2+/H2O2.Conclusion: OPS-Ia has a protective effect on membrane lipid peroxidative damage whichwas induced by H2O2 and Fe2+/H2O2. 3. The anti-diabetic effect of OPS-Ia on streptozotocin-induced diabetic miceMethods: KM diabetic mice were induced by a single intrapersonal injection streptozotocin(120 mg·kg-1). OPS-Ia was given to diabetic mice through gavage for 22 days. The anti-diabeticeffect of OPS-Ia (50,100,200 mg·kg-1) on diabetic mice were investigated by observing thechanges in body weight, water intake, food intake, the level of plasma glucose(PG), glucosetolerance, total cholesterol (TC), triglycerides (TG), high density lipoprotein (HDL-C), serumcalcium, plasma urea nitrogen (PUN). Moreover, the contents of hepatic glycogen andmalondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX),theglucose-6-phosphatase (G-6-Pase) activitities were also evaluated in diabetic mice liver.Results: Treatment with OPS-Ia significantly decreased the food intake, water intake, level ofPG, TC, TG, PUN, and MDA content in livers. Treatment with OPS-Ia significantly increased thebody weight, the content of hepatic glycogen, and the activities of SOD and GSH-Px in livers.Moreover, the activity of G-6-Pase also decreased after treating for 22 days.Conclusion: OPS-Ia could improve the disease symptom of STZ diabetic mice. It could alsoimprove the metabolic abnormalities of diabetic mice and delay the progression of diabeticcomplications.
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