The Study Of Tianma Gouteng Decoction Influence The Circadian Rhythm Of Blood Pressure And Clock Gene Per2,bmal1 Expression

Basic principles of hypertension treatment is prevention target-organ damage and drop of blood pressure. Abnormal blood pressure circadian rhythms is related to target-organ damage of hypertension and cardiovascular event, which is important pathogenic factors independent of blood pressure level. Blood pressure circadian rhythms of produce and maintain are relevant to the periodic clock gene expression. The expression level and rhythm changes of Clock gene Per2,bma11 which are body biological clock running the key genes direct adjust blood pressure circadian rhythms. Liver Yang hyperactivity hypertension syndrome is a common TCM syndrome type. Tianma Gouteng Decoction, representative prescription of calming liver and suppressing liver-yang, has a definite therapy effect for Liver-Yang hyperactivity syndrome(LYI-IS). Although hypertension syndromes essence and Tianma Gouteng Decoction mechanism research has made certain progress, but there are still many problems:â‘ Up to now, there is still not reported that using ambulatory blood pressure (bp leels, blood pressure variation, blood pressure circadian rhythms) index system evaluation herbal estimate Chinese herb compound in domestic.â‘¡t is lack of a Study of regulating the dynamic balance effect.â‘¢Mechanism study lack systematic and depth, the specific mechanism Cannot be accurate Interpretation. Therefore, this experiment investigate the curative effect of Tianma Gouteng Decoction from the adjustment of the clock gene bmall and per2 abnormal express rhythms caused by hypertension and its the possible role way by interference Angâ…¡-AT1 receptor signaling pathways.We establish hypertension induced Liver-Yang hyperactivity syndrome hyperactivitysyndrome(LYI-IS)rat model and Clarify adjust blood pressure circadian rhythms of mechanism and the possible role way by observing expression of Clock gene Per2,bmal1. 1 Hypertension liver Yang hyperactivity card animal blood pressure circadian rhythms features and Tianma Gouteng Decoction intervention role.(1)Hypertension induced Liver-Yang hyperactivity syndrome(LYI-IS)rat model.Hypertension induced Liver-Yang hyperactivity syndrome(LYI-IS)rat model were established by two-kidney one-clip and intragastric adminstration of Fuzi decoction. The result indicated that the achievement ratio and survival rate was respective 83.41% and 95.83%. So the method of model could be used in the experiment(2) Study on Tianma Gouteng Decoction adjusting model of rats blood pressure circadian rhythms role.24-hour ambulatory blood pressure were monitored by using wireless telemetry. Characteristic value of blood pressure circadian rhythms curve is analyzed by Chronos-Fit. The result indicated that Tianma Gouteng Decoction can adjust model of rats blood pressure circadian rhythms2 The study on Tianma Gouteng Decoction influence the mechanism of model rat blood rhythms(1) The effect that Tianma Gouteng Decoction adjusting angiotensinogenâ…¡rhythm of rat models plasma.The levels of plasma angiotensinâ…¡and catecholamine were measured with enzyme linked immunosorbent assays. Rhythm eigenvalues were used ealuation of the the levels of plasma angiotensinâ…¡and catecholamine. The result indicated thatTianma Gouteng Decoction can adjust rhythms of the levels of plasma angiotensinâ…¡and catecholamine of model of rats.(2) the influence that Tianma Gouteng Decoction adjust AT1 of rat modelsWe established the method of RT-polymerase chain reaction (PCR) to inspect MRNA AT1 expression level. The result indicated that Tianma Gouteng Decoction can adjust expression of AT1.(3) Tianma Gouteng Decoction to rat models, the influence of per2,bma11 expression.Establish RT-polymerase chain reaction (PCR) method inspected per2, bmall mRNA expression level bmal1,per2, the mathematical model were established. Rhythm eigenvalues were used ealuation of the influence of per2 bmall expression. Results showed that Tianma Gouteng Decoction influenced model rats per2,bmall expression.3 Blood pressure with the circadian clock gene correlation analysis. Using Chronos-Fit and LabFit program analysed experimental index, The result showed there is a correlation blood pressure circadian rhythms and clock per2,bmall genes.