Detect And Analyze On The Content Of Type Ⅰ And Type Ⅲ Collagen In Tissue Of Abdominal Wall In Adult Patients With Inguinal Hernia

Objective:To detect and analyze the difference of TypeⅠcollagen,TypeⅢcollagen, total amount of collagen(typeⅠ+typeⅢ) and the ratio of collagen typeⅠ/Ⅲthat origin from skin, anterior rectus sheath and transversalis fascia in adult patients with inguinal hernia and without hernia. Methods:we collect 20 patients with inguinal hernia and 20 patients with acute appendicitis operation(control group). Matched-pairs design was used based on ages,sex,tribe and history of smoking.To remove the factors that can induce abnormal metabolism of collagen.All patients' tissue specimens were obtained at the end of operation from skin, anterior rectus sheath and transversalis fascia. All specimens were confirmed by staining with H.E.The contents of collagen typeⅠ/Ⅲwere detected using immunohistochemical methods and the system of computer image analysis. The data were presented as gray level.The lower of gray levels and the more content of collagen in tissue. The total amount of collagen is sum of TypeⅠand TypeⅢ. Consequence data were expressed by x±s and statistical analysed by SPSS 17.0, size of test was a=0.05. To investigate the difference of TypeⅠcollagen,TypeⅢcollagen, total amount of collagen(typeⅠ+typeⅢ)and the ratio of collagen typeⅠ/Ⅲbetween two groups. Results: The inguinal hernia group had less typeⅠcollagen, more typeⅢcollagen, more total amount of collagen and lower ratio of typeⅠ/Ⅲthan control group. Conclusion:Inguinal hernia can be regarded as a disease of systematic collagen disorder and a local manifestation of a systematic collagen disorder.