Four-pin Method Of Adjuvant Arthritis In Rats Analgesic Effect And The Mechanism Of The Role Of The Hypothalamus

Objective: The study is designed to investigate comparatively on the analgesia after-effects of electro-acpuncture,filiform acupuncture,blood-pricking therapy,medicine injection therapy and their analgesia mechanism in hypothalami.Methods:Useing adjuvant arthritic(AA) rat as the model of inflammatory pain,selecting KUNLUN as the acupoint, adopting electro-acpuncture,filiform acupuncture,blood-pricking therapy,medicine injection therapy, using methods of radioimmunoassay,in situ-hybridization histochemistry,the study observed targets of the changing tendency of pain threshold, content of β - endorphin( β -EP) , adrenocorticotrophin(ACTH) and genie expression of pro-opiomelanocortin(POMCmRNA) in hypothalamus at the point of Oh,24h,48h,72h,120h after above-mentioned therapy have been finished.Results: All the used therapy have various degree of immediate and after-time analgesia effects.Electro-acupuncture's analgesia effect reached the peak immediately.which faded away 24 hours later and presented again at 48,72hour-points. meanwhile, β -EP raising,ACTH descenting and genie expression of POMCmRNA raising in hypothalamus.Same tendency occurred to filiform acupuncture with a comparatively moderate raised β -EP.The analgesia effect of medicine injection therapy lasted for 72 hours and arrived at its peak at 48 hour-point,the content of ACTH in hypothalamus descented correspondingly.The analgesia of the blood-pricking therapy disappeared 24 hours later then occurred again and lasted during the days afterwards.There're no evidence to prove that the realization of the analgesia effect of the pricking therapy was relative to the change of the β -EP, ACTH and genie expression of POMCmRNA in hypothalamus.Conclusion: Both the effect of immediate analgesia and that of after-time on inflammatory-pain have been observed in all four methords.In hypothalamus level,eletro-acupunture and miliform acupuncture maybe realized the effect of analgesia by raising the content of β -EP,reducing the content of ACTH,raising the genie expression of POMCmRNA .Medicine puncture therapy maybe have the effect of analgesia by reducing the content of ACTH. The change of the content of the above-mentioned substances were not observed after blood-pricking method was used,therefore there may be any other pathways to explain its mechanism .