Study On Mechanism Of Chinese Herbal Medicament Known As NFPL In Prevention And Treatment Of Dam Endometritis

Endometritis is one of common reproductive diseases in female animals, badly impacting the development of animal husbandry. Antibiotics and hormones have been the first priority for the prevention and treatment of this disease. However, the toxicity and side-effect of this therapy for food safety has been paid more and more attention although the therapy still works to some extent clinically. To avoid the toxicity and side-effect of these antibiotics and hormones used in veterinary clinic, the Chinese herbal medicine were developed for the purpose of the prevention and treatment of dam endometritis. Based on the traditional Chinese medicine theory, our research group successfully developed a Chinese herbal medicament known as New Fertility-promoting Intrautenne Infusion Liquid (NFPL) with definite and stable curative effects on endometritis. In order to further improve prescription and curative effects of this drug, the effects of NFPL and its component herbs in comparison with other Chinese herbs and additive prescriptions on growth of pathogenic bacteria caused endometritis, hemorheological parameters, endometrium structure and uterus motility were determined, and its mechanism was studied from the aspects of clearing away fever and drying dampness, activating blood flow and removing blood stasis, expelling putrefaction and eliminating pus in the present study.Experiment I Separation and identification of pathogenic bacteria caused endometritis in dairy cattle The experiment was designed for the preparation of pathogenic bacteria used for the detection of antibacterial activity with Chinese herbal medicament - NFPL and its component herbs, as well as for the establishment of pathological model of rabbit endometritis. The uterine secretion from dairy cow with clinical endometritis in Nanjing area was sampled and the pathogenic bacteria were separated and identified as routine protocol, resulting in 10 strains of Staphylococci, 6 strains of Streptococci and 8 strains of E. coli obtained. The serotypes of hemolytic and...