Study On The Mechanism Of Mongolian Bloodletting Therapy On Hyperlipidemia Model Rats

Hyperlipemia means the level of total cholesterol(TC), triglyceride(TG) or low density lipoprotein(LDL) in serum is excessive than normal.Lipid metabolic disturbance lead to cardio- cerebral vascular disease and damage the human health mainly. Cardio- cerebral vascular disease was named "first murder" to human health by WHO, and about 15 million people die of it every year all over the world. The morbidity of atherosclerosis in American is high, which belongs to vital cause of death and makes one-third proportion in annual death. The morbidity of adult hyperlipemia in American is 50%. The mortality of cardio- cerebral vascular disease in China is up to the first place today from seventh inin the 1960 s. The population having tendency of hyperlipemia is about35%. Excessive total cholesterol(TC)in plasma is an important risk factor to atherosclerosis(AS) which could increase the morbidity and mortality of cardio- cerebral vascular disease, and the patient with total cholesterol(TC)become younger in average age.Medicine research focus on prevention and cure of hyperlipemia recently. A lot of clinical research indicated blood-letting therapy is useful to hyperlipemia. The subject treated rats with hyperlipemia, observed the effect on Lipid metabolism, antioxidation, endothelium function and indexes relevant to liver tissue, discussed treatment mechanism to hyperlipemia, provided experimental proof to clinical research ontreating hyperlipemia with blood-letting therapy.The study consists of literature review and experiment. The former includes the mechanism of hyperlipemia in western medicine, the cause of it in Mongolian medicine, current therapy summary and application of blood-letting therapy in clinic.Experiment:Objective : To discuss the cure mechanism on blood-letting therapy for hyperlipemia, then provide experimental proof for prevention and cure on hyperlipemia and other relating diseases by the way of treating rats with hyperlipemia under blood-letting therapy. Method: 50 wistar rats(weighed 200 士 20)had an adaptation feeding for one week with general fodder before dividing into 5 teams with 10 rats in each group according to weight. The 5 teams included blank control team(A team), hyperlipemia model team(B team), blood-letting therapy team(C team), regular acupuncture team(D team) and positive drugs team(E team). Blank control team were fed with general fodder, other teams with fatty fodder. At sixth week, rats in blank control team(A team) and hyperlipemia model team(B team) were taken blood by cutting tails to test total cholesterol(TC),triglyceride(TG), low density lipoprotein(LDL) and high density lipoprotein cholesterol(HDL-C), count atherosclerotic index(AI) to make sure molding was successful. At seventh week, rats in blood-letting therapy team began to be bled(san zi soup were given to rats by intragastricadministration before bleeding for 3 days, one time a day; first bleeding at right total vein of Zang and Fu with volume of 0.5ml, second at left total vein of Zang and Fu with same volume), once a week for two weeks; Rats in acupuncture team(D team)were accepted acupuncture at Zu San Li point for 20 minutes once a day for two weeks. Rats in positive drugs team(E team) were given Zhi bi tuo(0.42g\kg.d), the ones given0.3%CMC-sodiumsolution every day in blank control team(A team).Many indexes were examined after therapy, such as TG, TC,HDL-C,LDL-C and NO in serum, high shear viscosity of whole blood,Low shear viscosity of whole blood, packed cell volume, red cell assembling index,plasma viscosity, erythrocyte electrophoretic time, activity of SOD, level of MDA and GSH-Px in serum and liver tissues, activity of ET,TXB2, 6 ■keto ■ PGFla, LPL, HL in plasma; the structure and shape of liver was observed by light microscope.Result:1.Index in serum post-treatmentTG,TC,LDL-C : There was statistical significance in comparison between C team and model team(P<0.01)as well as D team and E team were compared to model team independently(P<0.05). The result showed the teams of C/D/E could decrease TG,TC and LDL-C. The effect of C team was superior to D team and E team.HDL-C: The level of HDL-C in those teams of C,D,E was highersignificantly(P<0.05)than model team which showed the three teams could increase HDL-C. But no significance appeared among those three teams(P>0.05).2. Hemorheology Remarkably significant difference was found in the following indexes of high shear viscosity of whole blood, Low shear viscosity of whole blood, packed cell volume, red cell assembling index, plasma viscosity and erythrocyte electrophoretic time between C team and model team(P<0.01). The same indexes of model team were compared to D team and E team respectively, both comparison had significant difference(P<0.05). All results indicated blood-letting therapy team exceed acupuncture team(D team) and positive drugs team(E team).3.Antioxidation SOD, GSH-Px: The level of SOD, GSH-Px in teams of C,D,E were higher with statistical differences than these in model team(P<0.05), which showed the therapy in teams of C,D,E could increase SOD,GSH-Px;MDA: The levels of MDA in teams of C,D,E were lower than that in model team statistically with no difference(P>0.05) which showed only tendency of reduce.4.Fuiictionof Vascular endothelial ET: Rats in teams of C,D had lower ET level than that in model team with significant difference(P<0.05), especially evidently in comparisonbetween C team and model team which demonstrated C team was better than D and E team at reduce activity ofET.NO:Compared to model team,C,D team has significant difference(P<0.05),and E team had more obviously, showing that therapy in E team boosted NO over other two groups.5.giyceridase LPL,HL: Teams of C,D,E got higher growth than model team(P<0.05),which indicated the treatment of C,D,E teams could increase LPL, HL with no significance among those three teams(P>0.05).TXB2,6- Keto- PGFla:Although the three teams of C,D,E had no significant difference(P>0.05) compared to model team at decrease of TXB2, 6 ■ Keto- PGF la, but the standard value showed those three treatments had the potential to get them down.6.The liver morphology The teams of C,D,E could protect liver tissue comparing to model team with significant difference(P<0.05).Conclusion:1.The blood-letting therapy of Mongolian medicine could lower TC,TG,LDL-C in serum, raise HDL-C when hyperlipemia accompanied,which showed the method played a good role in lipid metabolism. 2.The blood-letting therapy could make influence on hemorheology effectively,to speed up the blood flow velocity, prevent atherosclerosis and Cardio-cerebral vascular disease, 3.The therapy could activate Antioxidant enzymes, ease Lipid per oxidation through testing SOD,MDA,GSH-Px in serum and liver.4.The therapy could ease damage to blood vessel endothelium, prevent and keep from AS and CHD by means of affection on ET and NO.The result played a great role in precaution and reduction of AS and CHD 5.The therapy could add LPL,HL, adjust blood lipid protease in order to change lipid metabolism. 6. The therapy could improve metabolic function of liver fat cells and make further efforts of protection.