| Purpose:Retinal vein occlusion is a common clinical retinal vascular disease, Mainly due to retinal blood circulation caused by abnormal retinal vein circumfluence suffocate, cause retinal hemorrhage, edema, exudation and a series of fundus pathological change. The disease causes complex, long course of disease, acute onset,severe visual impairment,is second only to diabetic retinopathy second large retinal vascular disease. The common symptoms for patients with decreased vision and even blindness due to complications. More common in older patients, but also have young patients. Retinal vein occlusion(CRVO), central venous obstruction, and branch vein occlusion(BRVO), according to the location of venous obstruction. Because of the severity of different, divided into ischemic and non ischemic, schemic lesionsthan the latter to be serious.The cause of the disease is complex, and there are many risk factors.No specific treatment of Western medicine, Mainly aimed at pathogeny and prevention of thrombosis, and active treatment ofcomplications, Such as lowering blood pressure and intraocular pressure, reducing blood viscosity, improving microcirculation, reducing thrombosis and tissue edema, prevent the formation of new blood vessels etc. Treatment is mainly thrombolysis, hormone drugs combined with laser photocoagulation, surgery treatment, the treatment effect is not remarkable, and part of the treatment of disputes more.In traditional Chinese medicine, retinal vein occlusion according to the cause of vision loss inthe extent and amount of bleeding were attributed to "sudden blindness", "shizhanhunmiao", "the cloud moved eye" etc.The pathogenesis is mainly the failure of the liver procurement,phlegm stagnation,blood stasis group network; Liver and kidney yin deficiency, yin deficiency and yang hyperactivity, causing the blood against the chaos, blood does not follow through on goal, overflow in the eye; deficiency of both the heart and spleen, Qi and blood is not sufficient, choroidal dystrophy, intraocular collaterals blood stasis due to control or bleeding,Belongs in the category of traditional Chinese medicine blood disease. In the theory of Zang Fu organs, because of the "The eye is the body opening of the liver" "The liver and the kidney have a common source" and "pupil" in "Five round theory" belongs to the kidney water,and closely related to liver and kidney, treatment to Ning blood stasis as the main therapeutic principle.Traditional Chinese medicine preparation for promoting blood circulation and removing blood stasis in traditional Chinese medicine to improve microcirculation,but not suitable for all types, and easy to cause retinal vein bleeding.The clinical need for non-invasive, effective, easy to accept treatment.Eye acupuncture therapy was founded in the 70’s of the last century, the inventor of the old professor Peng Jingshan.Eye acupuncture theory from the "Neijing" and "Rule of evidence", The eye is divided into eight zones of thirteen points. With the characteristics of acupuncture needle to small needle, less acupoints, acupuncture, manipulation of shallow light, simple operation, quick effect, has been applied extensively in clinical therapy based on stroke neurological department of internal medicine disease and pain disorders, and received obvious effect, In recent years clinical application in the treatment of disease gradually widening, and has obvious effect. Experiments have shown that eye acupuncture can improve the ischemic area in patients with cerebral infarction blood flow, functional activities stimulate brain cells, increase local blood flow. In recent years, through the 973 project study further proposed The core theory of the eye acupuncture "Eye acupuncture thirteen area eight points on the meridian in the brain, and the viscera", confirmed that Eye acupuncture with collaterals in the brain, through internal organs, blood circulation, activate collaterals function.Eye and brain are closely linked in physiology, brain is the "house of the original spirit", And "Eye is the opening of god", "The eyes like line connected to the brain and the things which are seen in the brain",Eyes can reflect the spirit of human consciousness status, Sensing the prognosis of disease. "The head is the meeting of Yang", the six Yang six yin meridians directly or indirectly in the Department of brain, And eye contact routes, Eight of the twelve meridians meridians to the eye as a distribution center.Also strongly linked to on the meridian of eye and the brain. Thus the eye and brain are closely related, physical coordination, pathology is also the mutual change.Eye for outside intruders, located in the head, and the brain are interlinked, so the needle dazzling weeks will have a direct effect on the treatment of encephalopathy. Anatomy of ophthalmic artery branch, directly to the carotid artery, carotid artery branch system belongs to the brain. The retina is the brain tissue or extension of nerve tissue. Retinal vein occlusion disease name of traditional Chinese medicine "sudden blindness" corresponding or "shizhanhunmiao" and stroke- cerebral veins closed resistance or cerebral hemorrhage in pathogenesis and symptoms similar.Retinal vein occlusion in the eye, Eye acupuncturedirectly effect on the eye, the eye through the meridians, blood stasis Ning role should be more direct. But Eye acupuncture in the treatment of diseases in Department of Ophthalmology, experimental and clinical studies and reported less, Therefore, the study on the animal model of rabbit eyes of branch retinal vein occlusion, Observation of Eye acupuncture acupoints: liver, kidney, The upper energizer District, Comparing traditional Chinese medicine Xuesaitong treatment and combined with xuesaitongtreatment.Comparison before and after treatment of retinal vein occlusion position change of blood flow, the change of platelet aggregation rate of blood vessel diameter change, Differences in the pathological sections, and the expression of related factor HIF-1α, NF-KB, to analyze the therapeutic effect and mechanism of Eye acupuncture.Materials and methods: Experiment 1:Take 50 only 4 months old, weight 3.0±0.2 kg of health chinchilla rabbits were randomly divided into 5 groups, Each group of 10,selected right eye for experiment.The rabbits divided into group A: blank group, group B: model group, group C: Chinese traditional medicine group, group D: eye acupuncture group, group E: eye acupuncture plus Chinese medicine group.Intravenous injection of Rose Bengal xenon laser irradiation combined with preparation of branch retinal vein occlusion model by photochemical method, By slit lamp branch vein incomplete obstruction after confirmation, The treatment group of three groups were treated by intravenous injection of xuesaitong, the treatment of eye acupuncture acupoints: liver, kidney, The upper energizer District, eye acupuncture combined with Xuesaitong combined treatment, the above treatment once a day.On five groups of animal respectively in 1, 4, 7, 10, 12, 14 days of laser speckle imaging detection technology change detection for fundus vessel diameter and blood flow of thrombus. After 14 days of treatment in heart blood to detect ADP maximum aggregation rate and the removal of the eye for pathological examination and comparison of results.Experiment 2 :Take 30 only 4 months old, weight 3.0±0.2 kg of health chinchilla rabbits were randomly divided into 5 groups, Each group of 10,selected right eye for experiment.The rabbits divided into group A: blank group, group B: model group, group C: Chinese traditional medicine group, group D: eye acupuncture group, group E: eye acupuncture plus Chinese medicine group.Modeling and treatment of the same experiment 1, after 7 days, air embolism rabbits were harvested, immediately eye retina, separation, In the EP tube, the temperature of-80℃preservation, Expression of HIF-1α, NF-KBp-p65,NF-KBp65 measured by western blot protein, and the results of the analysis.Results:(1) Changes in vascular diameter fundus thrombus observation spot laser speckle imaging technique: Blank group blood vessel diameter has no obvious change. After the preparation of BRVO rabbit model, with the extension of time, the diameter of retinal vein blood vessels became thicker,In the model group, the diameter of the blood clots in the experimental animals was slowly increased, and the other groups were significantly different(P<0.01); Vessel diameter of traditional Chinese medicine group fundus thrombus significantly thinner, there is significant difference with the model group; The blood vessel diameter of the eye acupuncture group was thinner and the change of blood vessel diameter was significantly different than that of the model group, and no significant difference was compared with the traditional Chinese medicine group;Eye Acupuncture plus traditional Chinese medicine group of fundus thrombus significantly attenuate the most obvious, has the obvious difference with the model group and eye acupuncture group(P<0.05).(2) Laser variation in the speckle imaging observation of thrombus blood flow of fundus: The blank group with normal blood flow.After experimental animal model of BRVO preparation, fundus venous blood flow decreased significantly, thrombus blocking is serious,With the extension of time, the model group experimental animal parts fundus blood clots blood flow change is not big, blood flow is decreased obviously, compared with the other stable thrombus; The Chinese medicine group fundus thrombus blood flow was increased, there was significant difference with model group(P<0.01), With the eye needle group have no obvious difference; Eye acupuncture group of fundus thrombus blood flow increased, blood flow was higher, with significant difference compared to the model group; Eye acupuncture plus traditional Chinese medicine group of experimental animal fundus thrombus significantly increased, with the model group, Chinese medicine group, eye acupuncture group had obvious difference, the closest to the normal group, the blood flow recovered. (3) Light detection results: Blank group, without thrombosis. The model group, the visible red cell aggregation close adhesion and vascular wall, thrombus blockage seriously; The traditional Chinese medicine group, a small amount of red blood cell aggregation visible blood vessels, the agglomeration of red blood cells and vascular wall and no adhesion, thrombus blockage of lighter; Eye acupuncture group, intravascular red cell aggregation is less, thrombus blockage of lighter; Eye acupuncture plus traditional Chinese medicine group,the blood vessels in a small amount of red blood cell aggregation and thrombosis significantly reduce.(4) The rate of platelet aggregation results: The normal maximum aggregation rate of blank group. The maximum aggregation rate was significantly higher in the model group, there was significant difference compared with the blank group. The traditional Chinese medicine group, eye acupuncture group, eye acupuncture plus traditional Chinese medicine group after treatment, the maximum aggregation rate significantly decreased, there was significant difference with model group.No significant differences between three groups.(5) HIF-1α expression in each group: very little expression of HIF-1α in the retina of the blank group animal model of retinal tissue, The model group rabbit retina tissue HIF-1αcontent increased significantly, compared with blank group there were significant differences(P < 0.01),The traditional Chinese medicine group and eye acupuncture group HIF-1α levels compared with model group, have significant difference(P < 0.05), no significant differences between groups; eye acupuncture plus traditional Chinese medicine group HIF-1α close to no expression differences,and there was no significant difference with the blank group, model group and Chinese traditional medicine group, eye acupuncture group had significant difference(P < 0.05).(6)The expression of NF-KBp-p65 in each group: Blank group only a small amount of p-p65 expression in rabbit retina tissue,The model group p-p65 contentincreased significantly, compared with blank group there were significant differences(p < 0.01), the traditional Chinese medicine group and eye acupuncture can decrease the content of p-p65, with statistical difference(P< 0.05),The eye acupuncture plus traditional Chinese medicine group can reduce the content, compared with eye acupuncture group and traditional Chinese medicine group, there were statistically significant(P < 0.05), Compared with the blank group, there was no significant difference.Conclusion:(1) Eye acupuncture can effectively improve the blood vessel diameter and blood flow changes in the branch retinal vein occlusion modeland improve local microcirculation.(2)Eye acupuncture can reduce the branch retinal vein occlusion in rabbits platelet maximum aggregation rate.(3)Eye acupuncture can down regulate the expression of branch retinal vein occlusion of rabbit model bearing HIF-1α, NF-KB and blocking the pathway, and thus reduce the interaction between HIF-1 α,NF-KB pathway, preventing inflammation caused by the vicious spiral.(4)The curative effect of eye acupuncture treatment of branch retinal vein occlusion, is through the improvement of microcirculation, reduce platelet aggregation rate, improve the retinal ischemia and hypoxia state, blocking the inflammatory reaction pathway inhibit inflammatory reaction to play a role, combined with Xuesaitong treatment can achieve better effect. It is indicated that the mechanism of treating retinal vein occlusion by eye acupuncture is a multi angle and multi way.
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