| Purpose:This experiment by detecting kidney Yang deficiency model rats with heart failure after myocardial infarction heart heart colour to exceed index(LVIDd, LVIDs, FS, EF) and brain natriuretic peptide(BNP) expression level comprehensive evaluation in the rat cardiac function, observing Wu Shang in heart failure rat serum levels of matrix metalloproteinase 9(MMP- 9) and matrix metalloproteinases inhibitory factor 1(TIMP- 1), soluble ST2(s ST2), IL33 and myocardial tissue Ang Ⅱ, the influence of ALD, AT1 protein level, evaluate its efficacy, to explore the molecular mechanism of Zhenwu Decoction treatment of heart failure, explore heart failure mechanism of the improvement of the ventricular remodeling process center, rich Wen Yang Li Shui treatment of traditional Chinese medicine, at the same time provide clinical syndrome differentiation treatment to Zhenwu Decoction scientific experimental data and theoretical basis.Material and method:Selected 80 healthy male wistar rats as the research object, randomly selected from 10 to blank group, for the rest of the 70 by coronary artery ligation, 10% chloral hydrate produced by intraperitoneal injection of 0.3 ml / 100 g for anesthesia, after anesthesia in rats and fixed on the operating table, connection of animal auxiliary breathing breathing machine, rib in 3, 4, layered cut between the chest, with no damage to the anterior descending coronary artery ligation of suture needle, layered suture chest, postoperative intraperitoneal injection of penicillin for 3 consecutive days, after waiting for wound healing, day an exhaustion type cold water swimming, swimming the longest time for 30 min, or rat oral or nasal 10 s stop swimming under water, for 15 days, after swimming to the dry towel but without heating.Cause of heart failure after myocardial infarction heart model of kidney Yang deficiency. The building after the success of survival 50 rats were randomly divided into true Zhenwu Decoction high dose group, middle dose group, low dose group, western medicine group and model group 5 groups, each group of 10, only be Zhenwu Decoction high-dose group respectively is 37.8 g/content(kg/d), dose group was 18.9 g/content(kg/d), low dose group is 9.45 g/content(kg/d), are made from 6 ml, twice a day to fill the stomach, every time 3 ml, for 15 days. Western medicine group with captopril 10mg/kg, d) to fill the stomach, are made from 6 ml, twice a day to fill the stomach, every time 3 ml, for 15 days. The blank group, model group 3 m L/time to physiological saline, to fill the stomach, twice a day for 15 days. Part 1: By ELISA method determination of heart failure in rats serum brain natriuretic peptide(BNP), matrix metalloproteinase 9(MMP- 9), matrix metalloproteinases inhibitory factor 1(TIMP- 1), soluble ST2, IL33(s ST2). Part2:Using Western blot method method in the determination of myocardial tissue Ang Ⅱ, ALD, AT1 protein expression. SPSS 17.0 software was used to statistical analysis, the test data results with mean + /-standard deviation((x±s) said. Compares the single factor analysis of variance between groups, if the variance is using Dunnett test, P < 0.05 significant differences in indicators for results.Results: 1. Using the anterior descending coronary artery ligation, successful cause heart failure after myocardial infarction heart model of kidney Yang deficiency rats. 2. Heart exceeds the FS and the EF values: compared with normal group, model group, low dose group, middle dose group of FS value and the EF value were significantly lower(P < 0. 01), Chinese medicine high dose group, western medicine group there was no statistically significant difference compared with normal group(P > 0. 05), high dose group, western medicine Chinese traditional medicine the FS value and the EF value significantly increased compared with model group(P < 0. 01), explain the rat cardiac function is impaired, ventricular remodeling, in heart failure rat model induced by building success, Zhenwu Decoction high dose group and captopril group curative effect is obvious. 3. The serum brain natriuretic peptide(BNP level change: compared with normal group, model group, western medicine, Chinese medicine BNP levels were significantly higher in the lower dosage treatment groups(P < 0. 01), with statistical significance; Compared with western medicine group, high dose group BNP levels increased significantly(P < 0. 05), withstatistical significance; Group of western medicine and traditional Chinese medicine(TCM) and low dose group, middle dose group compared differences no statistical significance(P > 0. 05); In heart failure rat model induced by making mould, success and show the Zhenwu Decoction can improve heart function to a certain extent, Zhenwu Decoction curative effect of high dose group was obviously better than that of the captopril group. 4. The serum level of MMP- 9 and TIMP- 1: compared with normal group, each group the level of MMP- 9 was higher(P < 0. 05), model group, western medicine, Chinese medicine TIMP- 1 level drops in the lower dosage treatment groups(P < 0. 05), with statistical significance; Compared with model group, Chinese medicine dose and high dose group of MMP- 9 levels decreased(P < 0. 05, P < 0. 01), TIMP- 1 were significantly increased(P < 0. 01), with statistical meaning righteousness; Than relatively to the western medicine group, high dose group of MMP- 9 were significantly decreased(P < 0. 01), TIMP- 1 were significantly increased(P < 0. 01); That MMP- 9 and TIMP- 1 level associated with the degree of heart failure, true Wu Shang curative effect of high dose group was obviously better than that of the captopril group. 5. The level of serum ST2, IL- 33: compared with normal group, each group ST2 levels were significantly higher(P < 0. 01), with statistical significance, IL- 33 model group were significantly increased(P < 0. 01), with statistical significance; Normal group and high dose group, middle dose group there was no statistically significant difference comparing IL- 33 level(P > 0. 05); Compared with model group, western medicine, Chinese medicine high dose group, middle dose group ST2, IL- 33 were significantly reduced(P < 0. 01), with statistical significance; Western medicine and traditional Chinese medicine(TCM) of high dose group, middle dose group of comparison difference has no statistical significance(P > 0. 05); ST2, IL- 33 level associated with the degree of heart failure, in the Zhenwu Decoction curative effect, high dose group and captopril group no statistical difference(P > 0. 05), and a significant curative effect(P < 0. 01). 6. Myocardial tissue Ang Ⅱ, ALD and AT1 level: groups of rats myocardial tissue Ang Ⅱ, ALD and AT1 level were higher than in normal group(P < 0. 01), western medicine group and Zhenwu Decoction middle and high dose group can decrease the rat Ang Ⅱ, ALDand AT1 level(P < 0. 05), Zhenwu Decoction high dose group reduce rats Ang Ⅱ, ALD and AT1 level is the most significant(P < 0. 05), and Zhenwu Decoction dose group of Ang Ⅱ levels than has obvious trend(< 0. 01).Conclusion: 1. By using the method of coronary artery ligation combined with exhaustion swimming, copied heart failure rat model of kidney Yang deficiency syndrome(heart); 2. Zhenwu Decoction can improve heart failure rat heart EF and FS value, reduce the serum BNP level, improve heart function; 3. Zhenwu Decoction can significantly reduce heart failure rats serum MMP- 9, improve the level of TIMP- 1; That Zhenwu Decoction could inhibit the degradation of extracellular matrix(ECM), reverse the ventricular remodeling; 4. Zhenwu Decoction can significantly reduce heart failure rats serum s ST2, level of IL33 show Zhenwu Decoction by IL- 33 / ST2 L signal pathway, improve ventricular remodeling, and treatment of heart failure in rats. 5. Zhenwu Decoction can significantly reduce heart failure rat myocardial tissue Ang Ⅱ, ALD and the expression of AT1 protein, illustrate the potential through regulating Ang Zhenwu Decoction Ⅱ, ALD and protein expression of AT1, restrain the activation of RAAS system, thereby reversing ventricular remodeling, improve heart function.
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