The Study Of Inflammatory Mediator Releasing From Adipose Tissue And Their Mechanism Of Effect

Background: Increasing experiments and clinical studies show that obesity is associated with cardiovascular disease. Obesity is accumulation of excess adipose tissue. Studies show that adipose tissue secreting many kinds of inflammatory mediator plays a important role in onset and development of atherosclerosis, but the mechanism is unclear. Th injury of endothelium function is the earliest steps in atherogenesis. There is less reports about injury of adipose tissue on endothelium. In recent years more and more studies accumulate on that statins have anti-inflammatory properties independent of their cholesterol-lowering effects. For what is said above, we analyse the components of the adipose tissue cultured media and detect the direct or indirect effect of the media on endothelium, then intervene with some drugs. It will be very important to knew the association between obesity and atherosclerosis and to prevent from atherosclerosis.Objectives: To detect the inflammatory mechanism of atherosclerosis induced by obesity.Methods: To exam IL-6 and PAI-1 level releasing from adipose tissue. To detect expression of intercellular adhesion molecular (ICAM-1) in protein level and mRNA level of material released from dipose tissue on endothelial cells and the effect of adipose tissue on HepG2 and that CRP on endothelial cells, than intervene with drugs such as atorvastatin.Results: 1. IL-6 and PAI-1 level released from omental adipose tissue are higher than that from subcutaneous fat. IL-6 level secreted from omental and subcutaneous fat is correlated with BMI (r=0.85, 0.69, P<0.01, respectively), PAI-1 level released from that is associated with BMI, too (r=0.63, 0.71, all P<0.01).2. The effect of CRP released from HepG2 stimulated with omental fat conditioned media is stronger than that stimulated with subcutaneous fat conditioned media.3. sICAM-1 releasing from HUVEC stimulated with CRP is dose-dependent.4. Expression of ICAM-1 in protein and mRNA level of adipose tissue on endothelial cells is stronger than control. The effect of omental adipose tissue culturedmeelia is stronger than that of subcutaneous.5. Atorvastatin can suppress the expression and release of inflammatory mediator by adipose tissue and endothelial cells.Conclusion: 1. adipose tissue can release inflammatory mediator.2. Substance receased from adipose tissue can induce endothelial cells producing inflammatory mediator.3. CRP released from HepGa stimulated with adipose tissue cultured media and CRP damaged endothelial function.4. inflammation induced by omental adipose tissue is stronger than that by subcutaneous adipose tissue.5. Atrovastatin suppress expression of inflammatory mediator. It has an effect of anti-inflammation.