| The fund of this study (No 2001-004) is supported by the 211 project of national key subject of combination of traditional Chinese and western medicine, mainly probing the mechanism and material basis of banxia(BX) , shengjiang(SJ), gancaoxinxin(GC) decoctions for the gastric motility .Theoretical study:A review of study on analogous preparations ,material basis of compound prescription and gastric motility was summarized to provide a tactics of study on compound prescription and its analogous. In this study we chose BX, SJ, GC decoctions as the object to explore their material basis and mechanism for the gastric motility.Experimental study:PartiObjective: To observe the capacity of gastric evacuation of rats by given BX, SJ, GC decoctions and explore the mechanism of these decoction for gastric motility.Results: A promotive effect of BX decoction on the gastric evacuation was observed; a inhibitive effect of SJ decoction on the gastric evacuation was showed .a trend of promotion in GC decoction for gastric evacuation appeared.These decoctions have different influence on the gastrointestinal hormones: there was a marked drop of the levels of VIP,SP and a rise of SS in rat serum in group BX decoction. The SJ decoction could raise the levels of MTL,GAS and SS in rat serum. The levels of MTL,GAS in rat serum were also increased in group GC decoction. Correlation analysis between Gastric residue of dextran blue-2000 and gastric evacuation showed: There was a negative correlation in group BX decoction between gastric residue of dextran blue and level of VIP in rat serum; There was a positive correlation in group SJ decoction between Gastric residue of dextran blue and levels of VIP and SS in rat serum; there was a positive correlation between Gastric residue of dextran blue and levels of MTL in rat serum.Calponin and caldesmon were both the important proteins regulating thecontraction of smooth muscles. This study showed these two proteins could regulate the status of smooth muscles of stomach. Maybe the regulating effect of BX, S J, GC decoctions on gastric motility was partly induced by calponin and caldesmon .the express of calponin and caldesmon all had decreased trend in group BX,SJ,GC decoctions vs control group . The results of histochemistry showed: the express content of calponin in group S J decoction was the first,group GC decoction was the second and group BX decoction was the last, the express content of calponin in group BX decoction decreased greatly vs group control, the express content of caldesmon in group GC decoction was the first ,group SJ decoction was the second and group BX decoction was the last, the express content of caldesmon in three groups were all decreased obviously vs group control. The results of PT-PCR was similar to the ones of histochemistry.Part 2Objective: To study the contractive effect of three decoctions-contained serum on antral smooth muscle cells .Results: Three decoctions-contained serum could arised different contraction of rat antral smooth muscle cells. Good contractive effect showed when given 5% final concentration of serum and reacted for 5 min. the degree of contraction in group BX decoction was the first, group SJ decoction was the second and group GC decoction was the last.Different peak fluorescent value of intracellular calcium in rat antral smooth muscle cells was observed in three decoctions-contained serum groups. The peak value in group BX decoction was the first, group SJ decoction was the second and group GC decoction was the last. Peak fluorescent value increased greatly in group BX decoction vs group control. The results of scanning continuously were as follows: three decoctions-contained serum could increase the fluorescent value of cells obviously.Three decoctions-contained serum could decrease the express of calponin mRNA and caldesmon mRNA in rat antral smooth muscle cells.Part3Objective:To observe the three effective constituents content of three decoctions in rat plasm and analysis the relationship between the effective...
|
PDF Full Text Request