| Chronic myeloid leukemia (CML) , a decease generated by tumor proliferation of multiple-stem cells, has the principal clinical symptoms as asthenia, weight losing, fever, bone pains, swollen liver , spleen and lymphatic nodes . The proliferated marrow is mainly the granular cell, which the middle and old infant stage of the neutral granular cells and reticular cells increases rapidly. Thirdly, oxyphile cells and basophile cells are easily observed, and W.B.C. of the phenomena of blood end transparently increases quickly. Fewer of the causes have proved, including the hypocrisy of chromosome rearrangement.Chronic myeloid leukemia (CML) belong to the category of "XULAO" ? "JIJU" , "XUEZHEN" etc. in traditional Chinese medical science. Modern traditional Chinese physician have shown the different ideas on pathogenesy of Chronic myeloid leukemia. Some scholars believed that the asthenia is the cause of the decease; some considers appositely the decease causes the asthenia; or some believe it is a mixture of asthenia with the sthenia virus. The sthenia virus includes the different fevers from the toxicity, the warmth, the phlegm, moisture, and the stasis toxicity. The healthy asthenia is from turbid phlegm, the weaker visceral function, the innate healthy insufficiency, or improper nutrition of postpartum, injured by motions; splenic disorder. Furthermore, it might be from the circumstance to influence the normal function of organs, to block the circulation of blood and energy smoothly. It might be from the virus to generate the fever or the heat to burn the blood and energy vein to cause the epistaxis. Chinese physician have often applied the traditional Chinese medicine herapeutical principle ,such as activate blood circulation to dissipate the blood stasis, cultivate the energy, expel toxicity, cool the heat, and supplementing qi and nourishing yin.At present, many experts and scholars discover some pathogenesis on study and research to the Leukemia: suffered Apoptosis inhibited have relation on certain type leukemia's occurrence, many chemotherapeutics can induce leukemic cell apoptosis. Modern pharmaco research indicated the traditional Chinese medicine maybe can induce tumor cell apoptosis,it base on the theory such as follows: 1. immunological regulation provocate the Tumor Necrosis Factor TNF);2. Chinese crude drug have the cell toxic action;3. raise the level of hormone in corpore;4. induce tumor cell differentiation. Now, less study and research on traditional Chinese medicine complex prescription but the clinical and empirical study proved the herb's definite effective on anti-tumor, mean while ,the research on it's mechanism just on the starto Many study on herbs extractive, especially about the arsenical have revoked the scientist that Induce apoptosis is an important mechanism on anti-leukemia by traditional Chinese medicine, as same as other herbs extractive :elemene> garlic oik curcumine, harringtonine^ homoharringtonine (HH ) > indigo naturalis^ indirubin and meisoindigo etc. But all of them just limited on experiment stage. So, it is our responsibility to relieve patient's ailment which need study and research extensively and manufacture a kind of controllable Chinese drugs pharmaceutics with good effect and less adverse effect.In western medicine drugs for this disease ,many drugs be used such as Myleram carboxyuria, arabinosylcytosim ;homoharringtonine (HH)> 6-mercaptopurine(6-MP) ^ 6-thioguanine(6-TG), tribromomanicol > immunoreactive fibronectin(IFN) and signal transduction inhibitor STI-571. STI-571 can elective intercept the ATPand Abl kinase binding site, mean while inhibit the phosphorylation of hydroxyphenylaminopropionic acid residue which in BCR / ABL kinase enzymatic substrate, and mske enzyme inactivation so as to interrupt a series of signal transduction, but this drug cost too much.All treatment above-mentioned can't curing CML thoroughly, except bone marrow transplant, but it is also expensive.at same time, the donator is difficult to seek while company with serious side effect and high death rate recurrence rate.Chronic Myelocytic Leukemia (CML) is a kind of acquired character multipotent hemopoietic stem cell disease.Ph chromosome [t(9,22)(q34,qll) is distinctive genetics mark of CML, accordingly, it generate BCR/ABL fusion gene and transcribe to 210Kdafusion protein p210BCR/ABL, this protein reserve enzyme activity of PTK in origin oncoprotein C-abl. In chronic phase of CML,hematology characteristic display anomal distension on medullary system cell. Research have proved that growth rate of ancestral cell is lower than normal, while it's multiplication cycle prolonged. Numerous research indicate that BCR/ABL fusion protein inhibit apoptosis of leukemic cell so to lengthen the cell's live time. According above, apoptosis be inhibited is an important mechanism on CML, so the methods to induce apoptosis of CML cell became a consideration way to treat CML.On the basis of clinical experience of our tutor team, considering that pathogenesis are vicious toxin invade into marrow and disorder the blood, so to result blood stasis. Consequently appear many manifestation of blood stasis. XIAOYULIN is a kind of effective Chinese drugs pharmaceutics that based on many years clinical practice in our hospital. It can promoting blood flow > dissipate blood stasis. heat-clearing and detoxicating^ nourish YIN and support blood. Stasis in superficial venules and lymph vessels while middle-warmer energy to be hurted, toxin evil stay in xuefen for too long time .all above eveil will be harmful to body result to the symptoms of deficiency in QI and YIN, so base on XIAOYULIN, combine with Huangqishengmaisan to supplement QI and nourishing YIN which quoted from the classic ((Yi Xue Qi Yuan)) .In clinical research, we observe the Chinese Traditional medicine university of Guangzhou NO.l clinical subsidiary hospital blood department hospitalization department and outpatient. All is clinical definitely diagnosed to be the chronic granulocyte leukemia chronic phase( CML, CP), total 17.Based in Maintaining the quantity in the hydroxycarbamide, it is divided into treating group and control group, the former takes XIAOYULIN 8gs one time everyday,3 times everyday, add to take the Huangqishengmaisan medicinal broth 1 dose everyday. The control group takes XIAOYULIN 8gs one time everyday,3 times everyday.result show that two groups have the obvious difference in the medullary index and hemogram at treatment AP:PA , but both interclass have no obvious difference;two groups' clinical symptom and sings both have the obviously clinical symptom relief. XIAOYULIN plus Huangqishengmaisan to the symptom of deficiency of both QI and yin such as: weary spirt and hypodynamia> thirsty, pharyngeal dry -. feverish palms and soles, constipation, heart-spirit discomfort > hectic fever, nightsweat, tinnitus etc.the curative effect in symptomatic relief is obviously better than those group which use XIAOYULIN only, and the total integral calculus of curative effect XIAOYULIN plus Huangqishengmaisan group is also a better than those group which use XIAOYULIN only. It illustrates that XIAOYULIN plus Huangqishengmaisan have the more obvious advantage in improving the existence quantity of sufferer.In experiment study, apply the method of blood serum pharmacology to observe vitro XIAOYULIN and XIAOYULIN plus Huangqishengmaisan Chinese medicine compound in medicine-contain rabbit's blood serum proliferative kinetics influence to the cell line of K562 ,the empirical method adoption is MTT. The MTT effect show that the hydroxycarbamide is the quickest since the effect action, namely attains the inhibition rate of 38% or so in 24hs,it did not show the prominent difference in inhibition rate between 48hs/72hs and the 24 hs , P<0.05,it shows that the drug action of hydroxycarbamide has already achieved a climax at 24 hours, inhibition rates have obvious difference between hydroxycarbamide and other two groups at the 24hs, P<0.05, and the inhibition rate of XIAOYULIN plus Huangqishengmaisan is obviously higher than those use XIAOYULIN only.Three inhibition rates have no obvious difference among the three groups at 48hs, P>0.05, the inhibition rates are all in 40% or so.inhibition rates of hydroxycarbamide has no difference with other two groups at 72hs, P>0.05.But the inhibition rate of XIAOYULIN plus Huangqishengmaisan is obviously higher than those use XIAOYULIN only, P<0.05.the inhibition rate of XIAOYULIN plus Huangqishengmaisan group essentially achieve a climax at 48hs , and continue rising slowly after 48hs.It prove that hydroxycarbamide^ XIAOYULIN^ XIAOYULIN plus Huangqishengmaisan have obvious inhibition to the cell proliferation of K562 through this experiment. And the inhibition rate of XIAOYULIN plus Huangqishengmaisan to the cell of K562 is the topmost.Apply the TUNEL to examinate apoptosis circumstance of the cell line of K562 through vitro cultivation which is treated by medicine-contained blood serum , as to approach the possible mechanism that traditional Chinese medicine compound recipe treating the chronic myelocytic leukemia.The effect of TUNEL manifest the 3 groups medicine including hydroxycarbamide > XIAOYULIN and XIAOYULIN plus...
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