Studies On The Quality Standardization For Fructus Polygoni Orientalis

Prince's-feather fruit (Fruits Polygoni Orientalis) is the dried and ripe fruit of Polygonum orientale L., which belongs to Polygonum of Polygonaceae. It has the functions of dispersing stasis blood and mass gathering in the abdomen, relieving food retention and alleviating pain. Though it has been revised and reprinted for six times after the first recording in the Ch P in 1977's edition, its quality standard is still quite simple with only macroscopic description. Furthermore, the items, such as identification, assay, processing etc., are all blank. So it is quite difficult to control the inherent quality with the absence of evaluation criterion. In order to resolve the above questions, this research is provided.The systematical research is made on six chapters, including the literature, resource and commodity investigation, chemical constituent in effective parts, pharmacodynamics, extraction technology of reference substance, and the quality standard of classification and quantization. Even the quality-standard draft are made base on it.Serious problems are appointed to the investigation on 87 medicinal materials for commodity from 26 provinces (including the municipality directly under the Central Government and municipality). Some places use adulterant, such as the dried and ripe fruit of P. persicaria, P. hydropie, P. lapathifolium and Polygonum bungeanumserious in the same genus. Even the Cleistocalyx operculatus is used for the similar alias. Quality problem is more serious for the percentage of ripening of most commodities is only about 50%.The chemical constituents in its effective parts are confirmed for the first. Four chemical constituents from the acetic ether soluble part are confirmed as taxifolin, quercetin, 3, 3' -dimethyl ellagic acid and 3, 3' -dimethyl ellagic acid-4-O-β -D-glucopyranoside by their chemical and physical characters and data of spectrum. The constituent is firstly got from Polygonum plants, and 3,3' -dimethyl ellagic acid is firstly separated from Polygonum orientate L. The taxifolin and 3, 3' -dimethyl ellagic acid-4-O- β - D-glucopyranoside are set as its index compositions through specific study.For the first time, the anti-neoplastic pharmacologic action are sieved on four kind of passage cells of cancer of cervix, gastric cancer and liver cancer, for six kinds of crude extracts and two index compositions in the water extracts part, acetic ether part and n-butyl alcohol part of crude and processed materials in five concentrations and four cultivation times. The result is that the acetic ether part (the effective part),especially the two index compositions, is better in vitro cancer-restraining. Also the anti-neoplastic mechanism of them two has been studied firstly. The pharmacodynamics research and acute toxicity experiment are made about its function of removing stagnation food and alleviating pain for the first time, which provides credible reference for the clinical usage and new-drug development. Above all taxifolin and 3, 3' -dimethyl ellagic acid-4-O- ￡ - D-glucopyranoside are treated as its reference substances.The technology sieving is done for the reference substances for the first time, then provided the best extraction technology and made the foundation of industrial production and new-drug development.The quality standard draft is drawn up through technology study on classification and quantization. The approbation research is made on histomorphology, chromosome cytology and molecular biology for the first time. Even for the fist time, HPLC-fingerprint is researched by choosing single and double index compositions as reference respectively. The fingerprint's specificity and practicality are enhanced by double-index composition-scaling. For the first time, the inherent-quality evaluation standard is made by quantitative index. The best harvesting times and lower limit of taxifolin in commodity are provided by quantitative analysis of total flavones and two reference substances in different commodities and that of different harvesting times. All the above provide t...