Association Of HLA-DRB1,IL-4,IL-13,PAF-AH And High Affinity Receptor For Immunoglobulin E Gene Polymorphisms With Asthma In Children

Objective To investigate the association of HLA-DRB1,IL-4,IL-13,PAF-AH and FcεR I -βgene polymorphisms with asthma in children. Methods ELISA,PCR-RFLP,ARMS-PCR and SSP-PCR methods were developed in the study .X2 and t or u test were used in statistical analysis. Results The allele frequencies of HLA-DRB1*9 and *10 in asthmatic subjects were higher than those of controls.The allele frequency of HLA-DRB1*1 was converse.RR values were 2.58,2.43 and 0.25 respectively. The levels of serum totle IgE and IL-13 in asthmatic subjects were higher than those of controls.In severe asthmatic individuals plasma PAF-AH activities were lower than those of mild or moderate groups and controls.The allele frequencies of IL-4(-589C/T) and IL-13(Arg130Gln) gene mutations in asthmatic individuals were not different from those in controls.The LOG10IL-13 titer values were higher in asthmatic individuals with IL-13(Arg130Gln) gene mutation than those without gene mutation.LOG10 IgE titer values in group with IL-4(-589C/T) gene mutation were not different from those without gene mutation.LOG10 IgE titer values in IL-13 mutation homozygotes were higher than the IL-13 mutation heterozygotes and group without mutation.Allergen positive percentage were not different in asthmatic individuals with IL-4(-589C/T) and IL-13(Arg130Gln) gene mutation from those without mutation.The allele frequenceies of PAF-AH(G994-T) gene mutations in severe asthmatic individuals were higher than those in mild or moderate asthmatic individuals and controls.The plasma PAF-AH activities of PAF-AH(G994-T) gene mutation heterozygotes were half of those of controls and the plasma PAF-AH activities of homozygotes were almost absent.The allele frequenceies of FcεRⅠ-βI181L gene mutation in asthmatic individuals were higher than those of group without gene mutation,OR value was 2.00(95%CI 1.10～3.11). FcεRⅠ-βV183L gene mutation were absent in asthma -tic individuals and controls.The allele frequenceies of FcεRⅠ-βE237G and –109/T gene mutation in asthmatic individuals were not different from those in controls.LOG10IgE titer values were not different in asthmatic individuals with FcεRⅠ-βI181L and E237Ggene mutation from those without gene mutation.LOG10IgE titer values in –109C/T mutation homozygotes were higher than the –109C /T mutation heterozygotes and group without mutation.Allergen positive percentage were not different in asthmatic individuals with FcεRⅠ-βI181L,E237G and –109C/T gene mutation from those without gene mutation. Conclution Bronchial asthma is a complex genetic disease.HLA-DRB1*9,*10 and Fc εRⅠ-βI181L gene mutation were sensitive genes in asthmatic children. HLA-DRB1*1 gene was protective gene.PAF-AH(G944-T) gene was one of the candidate genes in severe asthma.IL-13 was important in asthmatic pathology.There was imbalace of PAF/PAF-AH in asthmatic children.IL-13(Arg130Gln) gene mutation was related with the level of serum IL-13.The levels of serum IgE were associated with IL-13(Arg130Gln) and FcεRⅠ-β-109C/T gene mutation homozygo -tes.PAF-AH(G994-T) gene mutation was related with plasma PAF-AH activity.