Influence Of Suramin Combined DDP On The Proliferation Of Human Nasopharyngeal Carcinomar Cell Lines(CNE-2)

Objective: To study the inhibition of proliferation of Suramine combined DDP on the cultured tumor cells of Human Nasopharyngeal Carcinomar Cell lines(CNE-2) in vitro which were tested by MTT assays and ATP-Bioluminescence assay. Gene expression difference of CNE-2 cells before and after inducing with Suramine and DDP were assayed with cDNA microarry techniques. And to explore its possible antitumor mechanism in vitro by induced-apoptosis and gene expression difference on tumor cells. Methods: The inhibition of proliferation of Suramine, DDP and Suramine +DDP was studied in different concentrations of Suramin (6.25, 12.5, 25, 50, 100 and 200μg/ml) and different time(24, 48, 72, 96 and 120h) on CNE-2 cells by MTT assay and ATP-Bioluminescence assay; The effect of Suramine induced-apoptosis on CNE-2 cells was observed in different concentrations by inverted phase contrast microscope, H.E stainning and transmission electron microscopy; the apoptosis rate was determined using flow cytometry(FCM) and TUNEL assay; the expression of vascular endothelial growth factor (VEGF) was detected by immunohistochemistry SABC. The effect of Suramin, DDP and Suramin with DDP on cell cycle distribution of CNE-2 cells was evaluated with flow cytometry(FCM) assay; gene -expression difference of CNE-2 cells before and after inducing with Suramin, DDP and Suramine with DDP were assayed with cDNA microarry techniques. Results: The effect of cytotoxicity of Suramin was found on CNE-2 cells. The growth of CNE-2 cells...