Research On The Deciduas Effect To The Luteal And Transforming Growth Factor-betal Expression Of The Kidney Deficiency Early Pregnant Women With Spontaneous Abortion And Herbal Medicine To Interfere The Serum TGF-β1 Of Aborting Rat Model

Objective:1. To study the Progestogen Receptor and Transforming Growth factor-beta1 expression in the deciduas so as to explore the mechanism of the kidney deficiency early pregnant women with spontaneous abortion.2. To examine the further mechanism of Pill ZHUN YUN III on kidney deficiency and the luteal inhibition aborting rat model.Methods:l.Research on the deciduas effect to the Luteal and transforming growth factor-betal expression of the kidney deficiency early pregnant women with spontaneous abortion: 23 patients were diagnosed as spontaneous abortion and the kidney deficiency in the first trimester in the 1st Affiliated Hospital, Guangzhou University of TCM. These patients' deciduas and the other deciduas from 40 women diagnosed as spontaneous abortion and the blood stasis ( or blood fever) in the same period as well as 40 normal early pregnancy women who desired conducting induced abortion were collected. AT the same time, their serum were collected.RT-PCR and LSAB were used to detect the expression of PRmRNA and TGF-β1 mRNA in the deciduas. RT-PCR also was used to detect the expression of TGF-β1mRNA in the PBMC.2. Study on the Herbal Medicine to Interfere the Serum TGF-β1 of Aborting Rat Model:SPF grade male SD rats, aged 10-12 weeks and female SD rats , aged 8-10 weeks.weight 200-250gm, were mated. Pregnant rats were daily fed with hydroxyurea450mg/Kg from the first day to the seventh day. daily for. On the eighth day, theywere fed with RU486 3.75 mg/kg to create a kidney deficiency and the lutealinhibition aborting rat model. The rats were divided into 5 groups:A."model withDuoLiMa,B: model without herbs, C: model with high dose herbal medicine,D: model with low dose herbal medicine ,E:controlled group(fed with salineinstead of medicine). There were 8 days for creating model and treatment.Different groups were observed at the same period for the weight, the rateof abortion, the lever of serum TGF-3 1.Results:1. On the gene or protein lever, the expression of PRmRNA and TGF-5 lmRNA inthe deciduas every group were different, the different wassignificant(P<0. 01), the expression of the kidney deficiency early pregnantwomen with spontaneous abortion was lowest(P<0. 01).2.Group A, B, C and D showed some symptoms of kidney deficiency, such aswithered fur, slow action and less ability. The body weight of rats in eachgroup were all increased from Dl to D8, the different wassignificant (P<0. 01), group E was the highest and group B was the lowest. Therate of abortion was decreased, the different was significant (P<0. 01), groupC and group A was lowest. Pill ZHUN YUN III could increase the serum leverof TGF-0 1.Conclusion:1.In the clinical research, the normal expression of PRmRNA and TGF-P lmRNAin the deciduas are important in maintaining early pregnancy. The lowerexpression is related closely to the kidney deficiency early pregnant womenwith spontaneous abortion.2.The expression of TGF-3 lmRNA in the PBMC and deciduas has the positivecorrelation.3. Pill ZHUN YUN III could increase the serum lever of TGF- p 1 of the kidney deficiency and the luteal inhibition aborting rat model. Pill ZHUN YUN III can regulate immune function.