The Mechanism Of Synovial Hyperplasia Of Rheumatoid Synovium

Hyperplasia of the synovial lining cell layer is one of the major morphologic feature of rheumatoid arthritis(RA).The cell numbers of two main types of synoviocyte, type A — macrophage-1ike synoviocyte and type B —fibroblast-like synoviocyte, have considerably increased. It has been identified that synoviocytes can cause severe joint destruction by secreting various cytokines, inflammatory medium and proteinases, which indicates its essential role playing in the pathogenesis of RA. However, the exact origin of the hyperplastic synovial lining in chronic synovitis is still a subject of controversy. Although some evedence suggests that new hyperplastic lining layer synoviocytes — type A synoviocyte are derived from macrophage/monocyte that have migrated from the blood, which is an important mechanism of synovial hyperplasia of rheumatoid synovium, certainly is not the only case.We aim to study the other possible mechanism of synovial hyperplasia of RA synovium by detecting some genes mRNA expression in synovium from RA, osteoarthritis(OA) and normal control, using dot blot, Northern blot, and in situ hybridization. (1) to observe the apoptosis - related genes—bcl-2 protooncogene,tumor suppressor genes P53 and Rb, expression in synovium.(2) to detect the mRNA level of proliferating - related genes —cyclin B1 and D3,TGF-β,in synovium. The study test the hypothesis of local proliferation...