Studies On The Tannin Compounds And Vitro Antineoplasmic Activity Of Rosa Davurica Pall. And Corylus Mandshurica Maxim. In Heilongjiang

The concd soln of the leaves of the Corylus mandshurica Maxim. and the roots of Rosadavurica Pall.,aq. Me₂CO homogenate were fractionated by column chromatography (CC)over Diaion HP-20 (14cmi.d.×55cm) with H₂O and aq. MeOH in a stepwisegradient-mode(H₂O-20ï¼…MeOH-30ï¼…MeOH-40ï¼…MeOH-50ï¼…MeOH-MeOH-[MeOH-H₂Oacetone(6:2:2)].The 20 constituents were separated and purified repeatedly and which were identifiedby ¹H-NMR, ¹³C-NMR, spectra and chemical methods. The separated chemical constituents are as follows: Davurica M1 (1), (+) -Taxifolin-3-O-β-D-apio-D-furanoside (2),Davurica D1 (3),Davurica D2 (4),Davurica T1 (5),Agrimonnin (6),Casuarictin (7),Catechin(8),Mandshurica l(9),Mandshurica 2(10),Heterophyllin A (11),Heterophyllin B (12),Heter-ophyllinC(13),HeterophyllinD (14),Heterophyllin G(15),1,2,3,6-tetra-o-galloyl-β-D-glucose(16),Tellimagrandinâ… (17),Tellimagrandiâ…¡(18),Hippophoenin B (19) and casuarnin(20).In which compounds 1,9 and 10 were isolated firstly as new tannins.The anti-tumor activities of compounds 1-20 were evaluated by MTT method. They allshowed potential anti-tumor activities on K562,HCT-15,HT-1080. The anti-tumor activitiesof these 20 compounds were researched for the first time in this present study.It was concluded that there were distributions of tannins in the leaves of the Corylusmandshurica Maxim. and the stems of Rosa davurica Pall. with the method of microscopicchemistry. The results show that tannins of the stems of Rosa davurica Pall. exist in corklayer, and tannins of the leaves of the Corylus mandshurica Maxim. reside in vascular bundle.