The Effect Of Mechanical Stimulation On The Adhesion, Invasion And Proliferation Of Salivary Adenoid Cystic Carcinoma

The purpose of this paper is to investigate the effects of cyclic biaxial mechanical stimulation on invasion capacity and proliferation relationships with expression of MMP-9 in the salivary adenoid cystic carcinoma (SACC) with high or low metastatic capacity. We observed the functions of mechanical stimulation in the invasion and proliferation of cells in vitro and investigated the mechanism in invasion of SACC.The purpose of this paper is also to investigate the effects of cyclic biaxial mechanical stimulation on adhesion of SACC cell-extracellular matrix (ECM) and expression of E-cadherin/catenin complex in the SACC high metastatic cell lines ACC-M, SACC low metastatic cell lines ACC-2,so we observe the functions of mechanical stimulation in the adhesion of SACC cell-ECM and investigate the mechanism in the adhesion of ACC.Low metastatic (ACC-2) and high metastatic (ACC-M) salivary adenoid cystic carcinoma cells were harvested. Mechanical stimulation were applied to the cells in vitro using a cyclic biaxial mechanical stimulation device, and the mechanical stimulation were applied to the cells for periods of 1,3 and 6 hrs every day, lasting for 2 days. The amplitude of mechanical stimulation applied to the cells was 4000μ. strain and 1000μ, strain, at a frequency of three hertz (3Hz).Unstrained cells were used as control. The proliferation of ACC-M cells, ACC-2 cells were studied with flow cytometry. The expression of MMP-9 and E-cadherin/catenin complex on the cell of ACC-M, ACC-2 were studied with laser scanning Confocal microscope (LSCM) and image analysis. SACC cell-ECM adhesion was assayed by MTT technique. Invasion capacity of ACC-2 and ACC-M cells was determined by transwell in vitro.The results showed expressions of MMP-9 and proliferation index on the cell of ACC-M higher than ACC-2. It revealed MMP-9 had close relation with invasion and metastasis of SACC. Expressions ofα,β,γcatenin on the cell of ACC-2 were higher than that ACC-M and E-cadherin on the cell of ACC-M were higher than that ACC-2 without mechanical stimulation. Cell-ECM adhesion on the cell of ACC-2 were higher than that of ACC-M without mechanical stimulation.Mechanical stimulation can change the expression of E-cadherin/catenin complex and MMP-9, invasion capacity, proliferation , cell-ECM adhesion on the cell of ACC-2 and ACC-M with the time. Mechanical stimulation can change the adhesion of the SACC cell and expression of E-cadherin/catenin complex of the SACC cell-ECM. We think it play an important role in metastasis of the cancer. Mechanical stimulation can regulate the invasion capacity and expression of MMP-9.It can promote metastasis of the cancer. In the present study we think that there are other proteins playing an important role in metastasis of the cancer.The date also proved mechanical stimulation can influence and regulate the growth of ACC-2 cells and ACC-M cells in vitro. It provides experimental evidence for advanced study on cellular mechanical research in oral cancer.