Experimental Study Of Integrated Traditional Chinese And Western Medicine On Intestinal Dysfunction By Intestinal Obstruction

Objective:The changes of intestinal mucosal barrier, nutritional status, immunologic function in intestinal obstruction rabbit model were investigated. And the effects of early application of Sijunzi Decoction on intestinal mucosal barrier, nutritional status, immunologic function after relieving obstruction was assessed.Method:After intestinal obstruction was made, the serum endotoxin, histopathological evaluation scores, the integrality of intestinal epithelium,levels of TP,ALB,Cr,PALB,TRF, IGF-1, IL-2/TNF-α, IgG, IgM, IgA, C3, C4 and T lymphocyte subsets were measured or observed by microscope in 24,48,72 hours. After relieving obstruction,the index above all were measured and was observed by microscope in 48,96 hours.Result:The serum endotoxin and Chiu's histopathological evaluation score were higher obviously in intestinal obstruction model groups than those in control group. The thickness of intestinal mucosa,the height of villus,the depth of recess and the surface area of villus were lower obviously. The levels of endotoxin and Chiu's histopathological evaluation score were significantlly positive correlated. Moreover,it and the surface area of villus were significantlly negative correlated. Levels of TP,ALB,Cr,PALB,TRF and IGF-1 were lower gradually.Especially PALB,TRF and IGF-1 were sensitive comparatively. Levels of PALB,TRF and IGF-1 were significantlly positive correlated. Levels of IL-2 was obviously lower. Levels of TNF-a,IgG,IgM,IgA,the percentum CD4+T cell and the ratio CD4+/CD8+were obviously higher after intestinal obstruction. Nevertheless,in intestinal obstrution 72 hours the percentum CD4+T cell and the ratio CD4+/CD8+ were obviously lower than in control group. The level of endotoxin and Chiu's histopathological evaluation score were obviously lower in the Sijunzi Decoction groups than in the natural recovery groups.The surface area of villus was obviously higher in the Sijunzi Decoction groups.Levels of TP,ALB, PALB,TRF and IGF-1 were obviously higher. The level of IL-2, IgG, IgM, IgA, C3 and C4,the percentum CD4+T cell and the ratio CD4+/CD8+were obviously higher in the Sijunzi Decoction groups. Moreover,the level of TNF-αwas obviously lower. Conclusion:The intestinal mucosal barrier was seriously destroyed after intestinal obstruction.The body was on negative nitrogen equilibrium to bring on protein malnutrition.The breakage of gut barrier, enterogenous bacteria and endotoxin translocation etc. activated immunologic system in the early intestinal obstruction. Concomitantly the intestinal was obstructed, immunologic system was destroyed. The Sijunzi Decoction can protect intestinal mucosal barrier function and regulate the movement and digest and absorption functions of intestinal tract to strengthen adaptability of intestinal tract and to improve spleen and stomach deficiency.And it can correct the disorder of immunologic system and improve immunologic function.