Nepeta, Wind Volatile Oil Anti-inflammatory Effects And Its Mechanism

Herba Schizonepetae and Radix Ledebouriellae is a piece of common Chinese herb of the relieving exterior category. It has the effect of removing Wind-cold by Dampness. According the theory of TCM,The anti-inflammation effect and the mechanism of the OSS was studied in the first time in this thesis by means of modern scientific research methods,such as analysis,immunohistochemistry and in situ hybridization at the level of cell molecule. The results of the experiments show that:1.OSS inhibited the 8 kinds of model of inflammation,such as acute,slow and supersensitivity inflammation model. These results suggested that OSS had a good effect of anti-inflammation.2.OSS had a marked suppressive effect on the normal and adrenalectomized mice's hind paw edema induced by Carrageenan. OSS could decrease the contents of prostaglandin E2 in various of kinds of inflammation model. It also could inhibited histamine,5-HT,NO and protein in inflammation tissue,increase SOD and GSH-PX. It had no apparent effect on either the weight of the rat's adrenal glands or the content of vitamin C and cholesterol. Its effects may be related to the inhibition on inflammatory mediators and do not depend on the pituitary-adrenal system.3.OSS was able to inhibited the content of interleukin 2,6 and increase IFN- Y in the rats induced by Freund's adjuvant. This result suggested the effect of the anti-inflammation of OSS may be related to cytokine.4,OSS decreased the expression of CD54 in the bronchial epithelium tissue;inhibited the activation of NF- K B p65 and expression of its mRNA in the bronchial epithelium tissue of acute lung injured rat. Therefore,the levels of pro-inflammatory cytokines were decreased.