Mechanism Of Puerarin Glucose Tolerance Factor Of Modern Compound Preparation Of Hypoglycemic Lipid-lowering Effects

The theory of Chinese Traditional medicine thinks that the incidence mechanism of diabetes lies in internal organs, regarding kidney as origin, laying equal stress on liver and spleen, relating closely with lung and stomach. Deficiency of energy and yin is the most familiar and basic mechanism of diabetes. Diabetes runs through top, intermediate and low internal organs, whose ill positions are top focus in lung, intermediate in spleen and stomach, low focus in liver and kidney. Kidney is thought as origin. In the late period of diabetes, as yin damages yang, leading insufficiency of yin and yang, diabetes turns to worse case. Though blood stasis symbol is general in diabetes, it is rare to cure diabetes by means of above methods in clinic. Therefore, nowadays it is meaningful to discover the essence of blood stasis in diabetes through clinical feature, basement of pathology and physiology, treatment methods and prescription, and to determine the reasonable application of Chinese-western medicine combination curing blood stasis in diabetes. The modern experimental researches indicate that glucose tolerance factor (GTF) is a kind of compound material of various amino acids centering with chrome and a vital adjusting factor for sugar and fat metabolism in organism, GTF also plays an important role in keeping normal level of glucose tolerant quantity. We integrated the achievement in and out of China to prevent and cure diabetes and high blood fat disease and practical clinical experiences to synthesize glucose tolerance factor (GTF) which has been generally known that is effective to diabetes. After that, we composed compound GEGEN capsule with GTF and valid composition, GENGEN element, from the traditional Chinese medicine GEGEN. This prescribe can benefit energy and spleen, living YIN and saliva, and raise blood and remove stasis. 1. SubjectThrough the research, we further explored the relation of diabetes (Dispelling Thirst) disease and blood stasis. According to the requests of pharmacy, i.e. " safe, effective, stable and control", and the theory of combining traditional Chinese and western medicine, we composed prescribes and used medicines to look for a kind of new medicine which has satisfied effect and is safer than chemistry synthesize medicine in preventing and curingdiabetes. We integrated biological pharmacy technique and traditional Chinese medicalpreparation effectively. The experiment is divided into totally five parts, of which maincontents and result as follows.2. Methods:2.1. Preparation of the compound GEGEN capsuleAt first, according to the requests of pharmacopoeia, we selected one kind of wild GEGEN, which is one of the seven medical GEGEN defined in. pharmacopoeia, as extract sample. And then, we determined the relative microstructure and relevant infrared and chrome content determination.2.2 The impact of compound GEGEN capsule to experimental diabetes small rats' empty stomach blood sugar and glucose intolerant quantity.In order to inquiry into the impact of compound GEGEN capsule to Alloxan diabetes small rats' empty stomach blood sugar and glucose intolerant quantity, to examine its effect on declining high blood sugar, the duplicated model young rats in diabetes are divided into model set, the sets that model sets fed the compound GEGEN capsule in big and small doses(fed the compound GEGEN capsule respectively 0.34,0.17 g/kg), the positive control set that model set fed Excellent Declining Sugar( fed Excellent Decline Sugar 5 mg/ kg), normal control set and model control set ig. Normal control set and model control set ig were given equal volume(0.2 ml/lOg) of distilled water once everyday for 10 days successively. Then we determined blood sugar and glucose tolerant quantity respectively.2.3. The impact of Compound GEGEN capsule to the blood fat level of big rat in high blood fat we took 50 Wistar big male rats and divided them into 5 sets randomly: normal control set(given normal foundation animal feed), High blood fat model se...