Studied Of Mechanism Of Action And The Trophism And Toxic Action Of Trace Element Boron On Rats

Boron,a kind of nonmetal element,with low content in crust.Boron has been used in industry,agriculture,animal husbandry,cosmetics,medical treatment and some other fields.It was showed that the quantum vis boron has trophic action on human beings and animals during the recent years.It was indicated by some animal experiment,high dosage of boron was bad for growth,development,metabolism and some other physiological function of men and animals,and even toxic function.In this experiment,192 rats,with 0, 40,80,160,320,640 mg/L boron in the drinking water,and the effects of boron on the growth and organ development of some organs,the blood physiology and biochemistry, the ability of anti-oxidation of serum and some organs,and the microstructure and ultrastrcture of tongue,stomach,liver,pancreas,sperm,parorchis,testicle spleen adenohypophysis were studied.At same time,it was told mechanism of action of boron nutrition and toxic function on microstructure and ultrastrcture level.And in this article, there was a certain dose range of boron nutrition and toxic function for rats initially.The concrete result was showed:1.Influence of growth and allelotaxy in rat by added to boronThe growth and allelotaxy were studied by detection of body weight,stem length and organic quality or length and calculation of Lee's and corresponding organ index. The results showed by added 40～320mg/L boron to drinking water,the BM,ADG,Lee's index,organ quality and intestinal length and index was not evidently influenced (p＞0.05) in rats,but there was a evident low in the quality of liver,adrenal gland,thymus and testicle of 65-days male rats(p＜0.05或p＜0.01).By added 40～80mg/L boron to drinking water,the most of organic quality in 95-days rats were not evidently rose(p＞0.05).By added 640mg/L boron to drinking water,the BM,ADG,Lee's index,organ quality and intestinal length and index was evidently influenced in rats(p＜0.05或p＜0.01).The results indicated that the growth and allelotaxy of rat were evidently promoted by added 40～80mg/L boron to drinking water,and evidently effected by added 160～320mg/L boron,and evidently repressed and even poisoned by added 640mg/L boron.2.Influence of physiological and biochemical indicator in rat by added to boronInfluence of physiological and biochemical were studied by blood cell count, hemoglobin level dectection,partial serum biochemical indicator dectection,blood smear and haemocyte morphological observeation.The results showed:compared with control group of same age,the reticulocytes percentage of each experiment group exhibited different degree increasing,the RBC numbers and Hb content of experiment groupâ… andâ…¡more raised significantly(P＜0.05),but the changes of the leucocytes percentage were more no significant(P＞0.05).The numbers of RBC and WBC,the content of Hb and the percentage of neutrophil,eosinophil and monocytic in experimental group fromâ…¢toâ…¤decreased significantly or most significantly(p＜0.05 or p＜0.01).Under the microscope, there were no obvious changers of all kinds of blood cells in experiment groupâ… .In experiment group fromâ…¡toâ…¤,with the boron supplementation increasing,the deformed and disintegrated grew in number.The pyknotic nuclei of lymphocyte could be observed,The granulations in granular leukocyte decreased obviously or even vanished, and the nuclei in them were stained hardly,especially in experiment groupâ…¤,some more segmented neutrophil with could be observed.The content of serum glucose in the experiment group was evidently lower than the control group on 65^th day,in which existed evident different in theâ…¢andâ…¤experiment group(P＜0.05或P＜0.01),the content of serum glucose in theâ…¤experiment group was evidently lower than the control group on 95^th day(P＜0.05).The activity of serum ALT in theâ…¢experiment group with 65-days and 95-days was evidently lower than the control group(P＜0.05).The activity of serum AST was evidently raise in theâ…¤experiment group with 65-days(P＜0.05), but lowed in theâ… andâ…¤experiment group with 95-days(P＜0.05).The activity of serum ALP was evidently lowed in theâ…¤experiment group with 65-days(P＜0.05).The content of serum Ca²⁺ was evidently rose in theâ…¤experiment group with 65-days,but evidently or extremely lowed in theâ…£andâ…¤experiment group with 65-days andⅡ～Ⅳexperiment group with 95-days(P＜0.05或P＜0.01).The content of serum phosphorus was evidently lowed in theâ…£andâ…¤experiment group with 95-days(P＜0.05).The content of serum Mg²⁺ in theâ…¢experiment group with 65-days andâ…¤experiment group with 95-days was evidently lowed than the control group(P＜0.05).The results indicated that biochemical indicators serum were influenced to different degree by added 40～640mg/L boron to drinking water.3.Influence of Boron on the ability of rat's serum and organs' anti-oxygenThe activity of SOD,CAT and GSH-PX was dectected by Coomassie light blue kit.The results showed it has no significant influence on each index of serum's anti-oxygen of rat by added 40mg/L boron into drinking water(P＞0.05).But activity of SOD,CAT and GSH-PX increased significantly of many organs of 95ds rat.Added 80 mg/L Boron,the activity of serum and SOD,CAT and GSH-PX of organs advanced notably on 95d,and the content of MDA of myelencephalon and liver depress significantly.Adding 160 mg/L Boron,activity of SOD of serum,hypothalamus,liver and pancreas,activity of CAT,GSH-PX of kidney and the content of MDA of pancreas and epididymal increase significantly.Content of MDA loss of 95d's rat's serum, hypothalamus and liver,SOD activity depress of myelencephalon and epididymis,and GSH-PX activity depress notably of myelencephalon,heart,lung and lymph node.Added 320 mg/L Boron,MDA content of serum of 65d's rat increases significantly,SOD activity of serum,liver,kidney,pancreas and spleen of 95d increase notably,many organs' MDA content and some organs' CAT and GSH-PX activity increase significantly.95d rat' serum and many organs' GSH-PX activity,SOD activity of myelencephalon and epididymal, CAT activity of liver and epididymal,depress notably.Added 640 mg/L Boron,it had significant or extreme significant influence of MDA content of serum and activities of SOD,CAT and GSH-PX of 65d and 95d rats.It had notably effect on 95d rat's organs content of MDA.Many organs' activities of SOD,CAT and GSH-PX lower than control group notably.The results indicated the anti-oxygen ability of body was enchanced to different degree by added 40～80mg/L boron to drinking water,but effected and even prisoned by added 320～640mg/L boron to drinking water.4.Effect of Boron on the microstructure and ultrastructure of rat's organsthe microstructure and ultrastructure of tongue,stomach,liver,testicle,spleen and adenohypophysis in 95d rat's organs show that:inâ… andâ…¡experimental groups, observation from light microscope,evey organ and tissue grow well,structures of each organ was clarity,cells arrange in rule.In cells,some organel such as Mitochondria,RER and golgi complex,were developed well,volume of extre cell in denohypophysis increased,number of secretory granules mainfolded,with high density.It showed us that appropriate content of Boron in drinking water can improved the structure and funciton of some organs obviously,when added 40mg/l,the effect was best.Inâ…¢experimental groups,it had some harmful or injury influence on the microstructure and ultrastructure of tongue,liver,pancreas and testicle in rat's organs,There was obvious damage effect on microstructure and ultrastructure of rat organs inâ…£andâ…¤experimental groups,It showed filiform papillae top of tongue dissolved,taste goblet shrunk back;stomach mucous membrane epithelium was necrosis and desquamated,fundic glands turned shrink back,structure in a mess;hepatocytes turned analosis or tumentia,the cell organs in cytoplasm decreased,and the structure was damaged in different degree,it showed orchiatrophy,the number of spermatogenic cells in contorted seminiferous tubules decreased significantly,epithelium on tubal wall was damaged terribly,and cell organs, such as mitochondria and golgi complex,etc.All those changes showed that,there would be bad and even toxic effect on development and structure of every organ in rat by supplement 320-640 mg/L boron in drinking water,and influenced function of organism and all organs terribly.