| Objective: In this research acupuncture treatments were given to the rats with spleen deficency asthma, and the influences of acupuncture at Zusanli on the morphological eosinophil apoptosis and Fas mRNA, Bcl-2 the mRNA expression were observed. The aim of this experiment was to explore that acupuncture treatment can regulate the inflammatory cell apoptosis and acupuncture is an effective method of treating asthma. It should be proved that acupuncture can play an important role in multi-aspects and at multi-targets so as to confirm the validity for treatment of asthma. The effective mechanism was studied in order to instruct clinic practice and optimize the scheme of selecting points to open a new field for Traditional Chinese Medicine and acupuncuture clinic research regarding asthma.Methods: The rat models with spleen-deficiency in Traditional Chinese Medicine (TCM) and bronchial asthma in West Medicine were set up. Under the guideline of the theory of TCM, after the rat models were established successfully, they were given acupuncture at Zusanli basing on the theory of reinforcing earth to strengthen metal. The concentration of TGF-βand GM-CSF in BALF were detected by enzyme linked immuoabsorbent assay (ELISA) and Apoptosis was detected by TUNEL.Fas mRNA and the Bcl-2 mRNA expressions in the tissue of lung were detected by hybridization in situ and The morphology of the lung tissue was observed with the light microscop.Results: Compared with control group (C), in both the combined disease with syndrome group (A1) and the asthma group (B1), the count of EOS increased and EOS apoptosis rates decreased (P<0.01), compared with the asthma group (B1), in group A1, the the count of EOS increased (P<0.01). The pathological result showed: there was inflammatory lesion of the bronchial tube and the lung tissue in different degrees, in both the combined disease with syndrome group and the asthma group; the inflammatory lesion of the bronchial tube and the lung tissue was reduced in both acupuncture groups. Compared with control group (C), in both the combined disease with syndrome group (A1) and the asthma group (B1), the levels of GM-CSF remarkably increased (P<0.01) and the levels of TGF-βremarkably decreased in BALF (P <0.01). Compared with the asthma group (B1), in group A1, the levels of GM-CSF remarkably increased (P<0.05). Compared with both the combined disease with syndrome group (A1) and the asthma group(B1), the levels of GM-CSF remarkably increased (P<0.01) in both acupuncture groups (A2,B2). Compared with the asthma group (B1), the levels of TGF-βremarkably increased in BALF (P <0.05).Compared with control group, in both the combined disease with syndrome group and the asthma group, the expressions of Fas mRNA remarkably decreased and the expressions of Bcl-2 mRNA remarkably increased (P<0.01 or P<0.05), compared with the asthma group (B1), in group A1, the expressions of Fas mRNA remarkably decreased and the expressions of Bcl-2 mRNA remarkably increased (P<0.01); At the same time, compared with the corresponding group A1 and B1, in both acupuncture groups (A2,B2), Bcl-2mRNA expression was remarkably reduced (P<0.01), Fas mRNA expression obviously increased (P<0.01). A positively significant correlation was found between the EOS apoptosis and the concentration of TGF-βin BALF in each group rats (r= 0.538,p<0.01). The results indicated a very significant negative correlation of the EOS apoptosis with the concentration of GM-CSF in BALF(r=–0.694,p<0.01); A positively significant correlation was found between the Fas mRNA expression and EOS apoptosis in each group (r=0.776,p<0.01); and a very significant negative correlation was found between the Bcl-2mRNA expression and EOS apoptosis(r=–0.804,p<0.01).Conclusion: Spleen deficiency can aggravate phlegmasia cell aggregation in peripheral blood of asthmatic rats, which accord with inflammatory reaction of airway on asthmatic organism. Inflammatory reaction of airway aggravated by spleen deficiency is through regulating factor density of inflammatory cell and related gene expression. Acupuncture at Zusanli could promote EOS apoptosis of rat models with asthma and asthma with spleen-deficency, and increase EOS superficial Fas mRNA expression of asthmatic rats with spleen-deficency, suppress EOS superficial Bcl-2 the mRNA expression and reduce concentration of TGF-β,GM-CSF in BALF. Acupuncture could reduce the inflammation of airway and lung tissue.
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