Research Of Clinical, Fundmental And Approach The Mechanism Of Biology Heat Transfer On Sand Treatment In Uyghur Medicine

Sand treatment is the specific therapy of Uyghur medicine which could eliminate abnormal balgham,promote blood flow and have an effect of anti-inflammatory.It is the comprehensive physical therapy consist of the heat transfer by hot sand,sun bathing,effect of magnetism,permeating of mineral matter and massaging of sand.which has more than one hundred years in Tulufan.The sanatorium of sand treatment has accepted six ten thousand patients(which come from abroad except for internal) been established in 1984 in Tulufan,Although the sand treatment has the distinctive curative effect in rheumatism and backleg pain,the method is fairly extensive in Xinjiang.The mechanism of action in sand treatment has not been understand.which waiting for us to reaserch and find the scientific and standardize schema on sand treatment.Meanwhile,because the sand treatment was confined by region,time,season,climate which cuts down the curative effect and affects application in clinic intensively.The study is a intersect research which makes use of the theory conduct heat and humor in Uyghur medicine to institutes the scientific and standardize schema of sand treatment,finds the adapted patients about sand treatment,sets up the platform of heat conduct of sand treatment indoor,Then reveals the mechanism of action and provide the scientific evidence for establishing theory system of sand treatment.Materials And Methods(1) we have set up the platform of monitoring sand temperature appling 2700 model multifunction data acquisition equipment which made in USA Keitley corporation, microcomputer,thermocouple and referencing freezing point.we have tested and measured the development changes with the times on the temperature,sand temperature in different depths,humidity of sand on the surface,illuminance and hardness.(2) 112 cases have adopted the therapy buried in sand who coming from sand treatment station to be in hospital in Xinjing Tulufan from 2007(according with diagnose of the osteoarthritis),continued fifth days.We have detected blood sedimentation, Creactive protein.meanwhile,early morning stiffness,swell,limitation of activity have been observed.All of the datas have been dealed with SPSS 13.0 software.(3) Available heat conductivity of sand is an important parameter with which simulates the heat transfer regularity from sand to body.The study has measured the Available heat conductivity of sand in Tulufan applying ball heat conduction,and has measured the changing regularity time-variation about the surface temperature of body and sand temperature.then has compared the results with the numerical value.(4) A model of genu OA had been established successfully by fixing right hind limbs with gypsum in New Zealand rabbits.26 of 28 animals were divided randomly into free movement group A and free movement added sand treatment group B.another 2 animals were used as models.these animals were treated by buried with sand once a day last twenty days.We has shoted the X-ray photograph in orthophoria and lateral position, observe the macrostructure of articular genu changes of cartilagineus cells and morphology after HE dyeing,the ultrastructures of Cartilage in their articular genua were observed under electron microscope,effectively.And detected the numerical value of hemodynamics and hemorheology,the serum and the synovial fluid of the diseased joints were obtained for the testing of IL-1β,TNF-a,NO;Positive expression rate of Caspase-3,Bcl-2,Bax were measured by immunohistochemistry and Terminal deoxynucleotidy transferase mediated dUTP nick end labeling(TUNEL).All of data have been dealed with SPSS 13.0 software.Result(1) Every year,during June to August between the dates of the 10^th through the 20^th, at the time 15:00 to 18:00 each afternoon it was determined that the air temperature of the sand treatment station reaching 40±2.3℃,and the sand used as a medicinal poultice could be maintained at the temperature of 38.±4.6℃at the depth of 20 cm from the sand surface.During the process of sand therapy,the skin surface temperature rises rapidly,up to 39℃,but the skin surface temperature falls subsequently as the heat stimulates perspiration and the cooling effect of evaporation takes place.When the skin surface temperature is 36±0.32℃it will fall to 36±0.6℃and maintain a stable temperature for two to three hours.When the sand temperature reached 43℃,it could not hurt the skin and tissue,because the body temperature could balance with sand temperature at an ideal temperature for the skin.(2) On the basis of the differentiation of symptoms and signs of Uyghur medicine,the propotion abnormal Balgham in osteoarthritis disease accounts to 53.57%,which is the most largest,the propotions of abnormal Khan,abnormal Spra and abnormal Savada account to 15.18%,10.5%,20.54%respectively.Sand treatment chould improve the symptom of the patient on early morning stiffness,joint pain and arthrocele(P＜0.008), Meanwhile sand treatment chould cut down the index of the laboratory on detected blood sedimentation,Creactive protein(P＜0.008).(3) The study has designed and measured the system of model experiment of sand treatment in door.And has measured the changing regularity time-variation about the surface temperature of body and sand temperature.then has compared the result with the numerical value.The results of the model experiment chould better reflect the tendency of temperature in surface.The results indicated that the temperature coincides comparing experimental platform indoor with outdoor According the traditional principle of the sand treatment.(4) A model of genu OA had been established successfully by fixing right hind limbs with gypsum.Sand treatment chould declined level of IL- 1β,TNF-a,NO in synovial fluid(P＜0.01).The cartilage tissue and cell cataplasia and ultrastructure changes were observed-in these animals right articular genu,It were observed that sand treatment chould improve inflammation,lessen the cataplasia of arthrodial cartilage.Sand treatment could improve Blood viscosity,depress peripheral vascular resistance(PVR) of the the rabbit experimental knee osteoarthritis(P＜0.01),meanwhile it could accelerate blood flow rate and lessen vascular resistance in arteria cruralis of the lower limbs in rabbit(P＜0.01). Sand treatment chould down regulation the express of the Caspse-3,Bax,Tunel in articular cartilage in KOA(P＜0.01).conculsion(1) We have Induced the standard of sand treatment including season,spot,time,temperature,depth.It makes sandtreatment more normalization,science and normalization.The ideal time for sand therapy is between June and August between the 10^th to 20^th days of the month,between 15:00-18:00 every day.When the air temperature reaches 40±2.3℃,the sand temperature will be maintained 38.±4.6℃under the 10-20. centimeter.Basised on this conditions the human body is buried under the sand at the depth of 20 cm for a period of one half of an hour to achieve therapeutic effects.(2) Abnormal Balgham is the major factor to cause the rheumatism.Furthermore, curative effect of the Abnormal is better than others.We consider that the most accommodative crowd is the patient of the abnormal Balgham.basised on the program,the curative effect of patients with disease of osteoarthritis has a significantly improved which includes the early morning stiffness,joint pain and arthrocele,Meanwhile sand treatment chould cut down the index of blood sedimentation,Creactive protein.(3) The system of conduct heat has been set up indoor which based on experiment method compared with the physiocracy in Tulufan.the temperature coincides to Tulufan outdoor.Furthermore,the system of conduct heat indoor of sand treatment will be not inhibited by the time,place,climate,and temperature will be controlled,(4) We have established the animal model of the osteoarthritis.Sand treatment chould declined level of IL-1β,TNF-a,NO in synovial fluid,The cartilage tissue and cell cataplasia and ultrastructure changes were observed in these animals right articular genu. Sand treatment could improve Blood viscosity,depress peripheral vascular resistance (PVR) of the the rabbit experimental knee osteoarthritis,meanwhile it could accelerate blood flow rate and lessen vascular resistance in arteria cruralis of the lower limbs in rabbit.Forthmore,sand treatment chould suppress the Apoptosis of articular cartilage, improve the inflammation.Meanwhile it made the proteinic express down regulation on Caspse-3,Bax,Tunel.So,we consider that This is one of the cause which it gives a play for anti-inflammatory action.(5) The study makes use of the biology conduct heat to research the sand treatment which provides a new method and means for research the sand treatment,and establishs theory basis for the reveal the mechanism action of sand treatment.