Mongolian Temperature For The Complex Mechanisms Of Fatigue In Rats

Chronic Fatigue Syndrome(CFS) is a unexplained syndrome mainly characterized by rave recoverable fatigue that are associated with multiple symptoms such as long term low fever,sore throat,headache,muscle and joint swelling and pain,blahs,forgetting,insomnia and sleepiness. In the Mongolian Medicine CFS was thought to the caused by physical, feeling (thought) and language activities of human body in the abnormal condition for long term.In this (disease) progression there are accumulating stress in the body, result in disorders of the nervous, immune and endocrine functions and lead to present a series of symptoms of CFS. This project focus on mechanisms the effects of Mongolian Medical warm Acupuncture therapy (MMWAT) on CFS,investigate the anti-fatigue effect and its regulate mechanisms on the fatigue in rat model using exhaustive swimming test plus tied methods and behavioral tests.OBJECTIVETo exposure the effects and its mechanisms of the Mongolian Medical Warm Acupuncture therapy on fatigue in rat modal.METHODSSD male rat, weigh 160-180g,60 rats are into 3 groups:normal control group (NC),fatigue modal group (FM) and warm acupuncture group (WAT); 20 rats in every group rats are feed by daily fixed feed stuff two times, feeding forl hour. before beginning of experiments.NC group:no any stimulation.FM group:Fatigue rat model was made by using exhaustive swimming plus tied methods to stimulate rats for 3 weeks.WAT group:do the same modeling process with Mongolian warm Acupuncture therapy using adding 100m A currents and 40℃temperature to the handtailor silver acupuncture needles through MLY-1 type Mongolian medical treatment method warm-pin Instrument an the selected Mongolian medical points-"Dinghui ", " Mingmai "and "Xinxue" (treat one time on one point in very 2d (day), earth stimulus (treatment) lasted for 15min, a total of seven times in 3 weeks). Fatigue mode; and effects of warm acupuncture therapy was evaluated by the ethalogy test, examine TNF-a, ACTH, C content in rat serum, NE,5-HT,DA in rat hyothalamus and free radical group in rat liver.RESULTS1.On rat body weight,there are no significant difference among the 3 groups before modeling experiments:end of the modeling experiments, there are significant difference in the between FM group and NC group, FM group rats lost body weight; body weight of WAT group rats are in the between of MF group and NC group.showed that stress induce loss of body weight in MF group rat. MF group rat compared with NC group, the number of walking and standing activities were increased. Shows that MF group rat presents physical and mental fatigue, warm acupuncture therapy can relief the stress and improve the physical and mental fatigue.In the modeling of 7d, struggling times of rats have no significant difference among the 3groups.In the modeling of 14d, MF group and WAT group rats compared with NC group respectively, the struggling times were increased and have significant differences. In the modeling of 21d, the model group,the warm acupuncture group compared with the comparison group, struggling times decreased,there are significant differences.In the modeling of 7d,14d,21d, the warm acupuncture group compared with the model group, exhaustive swimming time was significantly prolonged,there are significant differences.2.The MF group rats compared with NC group, content of TNF-α,ACTH and Corticosterone were in elevated and have significant differences.The MAT group RATS compared with the comparison with NC group, content of TNF-α,ACTH and Corticosterone content were reduced and have significant differences. The results indicate that the TNF-a, ACTH and Corticosterone in fatigue rats were significantly increased, suggesting that endocrine and immune function present disorders, and the warm Acupuncture therapy can improve the endocrine and immune function disorders.3. Compared with NC group, contents of NE,5-HT and DA in MF group rat were increased and have significant differences. Compared with the MF group, contents of NE,5-HT and DA in WAT group rats were reduced and have significant differences. These the result suggests that nervous function disorders are involved in the pathogenesis of CFS (fatigue) and warm acupuncture therapy can improve the nervous functions disorders.4. Compared with NC group, MDA content in MF group rats, tend to increase, but there are no significant differences.Compared with MF group, MDA content in WAT group rats tend to decrease, but there are also no significant differences. Compared with NC group,GSH content in MF group rats were increased and have significant differences Compared with NC group, GSH-PX content in MF group rats were increased significant differences. Compared with MF group, GSH-PX content in MAT group rats were increased and have significant differences;CONCLUSIONS AND DISCUSSION1.Previous Mongolian medical research described that CFS was caused by abnormal "Badagan" or "Hei", The present study first time exposure a new mechanisms of CFS onset that CFS was caused by the disorders of "three GenSu " that were triggered by abnormal "physical, thought and language" activities in body for long term.2. There are several ways to make the model of fatigue. Such as running, tied, and swimming. According to Mongolian medical theory, we proposed that CFS should be caused by disorders of " the three GenSu" that CFS should be triggered by abnormal physical, thought and language activities in body for long term,and that the Mongolian warm Acupuncture therapy should have a anti-fatigue effects. Therefore, we designed a alternative pathological model for CFS onset in rat that was combination exhaustive swimming and tied methods to induce fatigue.Studies have shown that swimming was more exhaustive than running. Mandatory swimming can induce animals despair and other psychological pressure, and more exhaustion. Therefore a mandatory swimming plus tied to stimulate and make fatigue model is more suitable the Mongolian medical theory and our proposal.3. In this study, long term stimulation of rats with exhaustive swimming plus tied methods induce abnormal physical, feeling activities, result in accumulation of stress. Accumulation of stress could induce disorders of nervous, endocrine and immune functions and lead to cause fatigue. These are well consistent with our proposal that CFS should be caused by the disorders of "the three GenSu" that were triggered by abnormal physical,though (feeling)and language activities. our proposal were also well supported by the results that Mongolian warm acupuncture therapy can improve the disorders of nervous, endocrine and immune function and relief the fatigue.4.DinghuiXue,MaiyongXue and XinXue 3 points that were selected for this experiment used to regulate the disorders of " the three GenSu". Clinical usage of the 3 points are well suitable for treatment of CFS (fatigue), indicate that CFS onset was due to the disorders of" the three GenSu". These further supported our proposal.5. Mongolian warm acupuncture therapy can regulate the disorders of " the three GenSu" and improve the disorders of nervous,endocrine and immune functions, and also improve the ability to defence of disease so that have a well anti-fatigue an CFS. This might be a possible mechanism underlying the anti-fatigue effects of Mongolian warm acupuncture therapy on CFS treatment.6. Well anti-fatigue effects of Mongolian warm acupuncture therapy could provide a new potential therapeutic and technique for clinical treatment of CFS .Moreover, Mongolian warm acupuncture therapy had many advantages and features such as simple, safety, effective and less toxic and side effects etc. Therefore, this study strongly recommend Mongolian warm acupuncture therapy to widely use in clinical treatment.