| Purpose: Analyze the theory from metabonomic side,by researching how He-Mu points effects on the serum and urine metabolic spectrums of the gastric ulcer mice.Method: Divide 72 Wistar mice into 6 groups: the normal group,the bundled group,the model group,"He-sea points"group,"Mu points"group,He-Mu points group.Gastric ulcer mouse model uses the water- immersion and restraint stress method to copy the model.The normal group shoud be only fed without any treatment;the bundled group shoud be bind everyday without acupuncture and copy model; the model group shoud be bind everyday and copied the model without acupuncture; the Mu points group shoud be acupunctured on"zhong wan"everyday and copied the model; the He points group shoud be acupunctured on"zu san-li"everyday and copied the model; the He-Mu points group shoud be acupunctured on both"zhong wan"and"zu san-li"everyday and copied the model. After 7 continuous treatments, observing the injuries of the gastric mucosa, analyze the differences between each groups, which the serum and urine metabolic spectrums of the gastric ulcer mice by LC-MS.Result:1 Visual observation on gastric mucosa,there are varying amounts and sizes of ulcer lesions appeared in the model group.Bundled group compared with the normal group has significant difference (P<0.01), showed bundle is also a stress stimulus; the model group compared with the bundled group has difference (P<0.05), showed the model had been successfully copied; the He-sea points group or the Mu points group compared with the model group has difference (P<0.05), He-Mu points group compared with the model group has significant difference (P<0.01), showed that simple acupuncture Zusanli or Zhongwan Point on the treatment of gastric ulcer model has some role in prevention and treatment, Mu points group,He-Mu points group compared with He-Mu points group has significant difference (P<0.01), showed He-Mu points group effect to surpass the He-sea points group, Mu points group.2 After the comparision of serum metabolic spectrum:There are 14 common peaks, 10 peak areas (No. 1, 5, 6, 7, 8, 9, 11, 12, 15, 17) have prominent difference between the normal group and bundled group(P<0.01). There are 16 common peaks, 5 peck areas (No. 4, 5, 12, 13, 14) have significant difference between the model group and bundled group(P<0.01, P<0.05), point out binding is also a kind of stress stimulation. There are 16 common peaks, 4 peak areas (No. 2, 3, 6, 8) have significant difference between the Mu points group and the model group (P<0.01). There are 16 common peaks, 4 peak areas (No. 2, 3, 7, 8) have significant difference between the He-sea points group and the model group (P<0.01), and No. 16 peak is a specific peak, may be a new product. There are 16 common peaks, 8 peak areas (No. 2, 3, 5, 6, 7, 8, 12, 13) have significant difference between the He-Mu points group and the model group (P<0.01), and No. 16 peak is a specific peak, may be a new product. Compared between the three treatment groups, there are 16 common peaks, 1 different peak, 5 peak areas (No. 2, 3, 6, 7, 15) have significant difference between the Mu points group and He-sea group (P<0.01, P<0.05); there are 9 peak areas (No. 2, 3, 5, 7, 8, 12, 13, 14, 15) have significant difference between the Mu points group and He-Mu points group (P<0.01, P<0.05). There are 9 peak areas (No. 2, 3, 5, 7, 8, 12, 13, 14, 15) have significant difference between the He-sea points group and He-Mu points group (P<0.01, P<0.05). Point out that acupuncture has effect on the serum metabolic products of gastric ulcer mice, the new peak and the peaks which have different peak areas may be the metabolic products are specific metabolic products of the prevention and treatment related to acupuncture the gastric ulcer mice serum. During three acupuncture groups , He-Mu points group has the most interrelated metabolic products. As a result, He-Mu points compared with single points has specific expression, there is a certain synergy.3 After the comparision of urine metabolic spectrum:There are 31 common peaks, 17 peak areas (No. 1, 3, 4, 7, 8, 10, 13, 15, 17, 18, 19, 28, 34, 37, 40, 41, 42) have significant difference between the normal group and bundled group (P<0.01). No. 9 and 20 are the new peaks, No. 16, 25, 33, 35, 38 peaks are the disappeared peaks, these special peaks may be the new products after the bundled stimulate. There are 30 common peaks, 16 peck areas (No. 1, 3, 7, 10, 13, 15, 17, 18, 19, 20, 21, 24, 28, 32, 40, 41) have significant difference between the model group and bundled group(P<0.01,P<0.05),and there are 12 metabolic products have difference with normal group and model group, point out binding is also a kind of stress stimulation. There are 34 common peaks, 12 peak areas (No. 1, 2, 6, 7, 10, 13, 15, 17, 20, 22, 23, 24, 29, 30, 32, 36, 37, 39, 40, 41) have significant difference between the Mu points group and the model group (P<0.01), No. 5, 31, 33 peaks are the new peaks, No. 28 peak is the disappeared peak, these metabolic products may be the new products. There are 35 common peaks, 13 peak areas (No. 2, 10, 12, 13, 17, 18, 24, 37, 40, 41) have significant difference between the He-sea points group and the model group (P<0.01), and No. 5 peak is a new peak, may be a new product. There are 31 common peaks, 10 peak areas (No. 2, 10, 12, 13, 17, 18, 24, 37, 40, 41 ) have significant difference between the He-Mu points group and the model group (P<0.01), and No. 5, 9, 16, 31, 33, 5, 38 peaks are the new peaks, No. 8, 15, 19, 22, 25 peaks are the disappeared peaks, these peaks are the specific peaks, may be are new products. Compared between the three treatment groups, there are 34 common peaks, 16 peak areas (No. 1, 2, 10, 12, 13, 17, 18, 21, 22, 23,24, 25, 29, 30, 37,40) have significant difference between the Mu points group and He-sea group (P<0.01, P<0.05), No. 28 peak is the new peak, No. 31, 33 are the disappeared peaks. There are 32 common peaks, 13 peak areas (No. 1, 2, 7, 10, 13, 17, 18, 21, 24, 32, 33, 37, 40) have significant difference between the Mu points group and He-Mu points group (P<0.01, P<0.05), No. 9, 16, 28 peaks are the new peaks, No. 8, 15, 19, 22, 25, 35, 38 peaks are the disappeared peaks. There are 31 common peaks, 11 peak areas (No. 1, 2, 7, 10, 13, 17, 18, 21, 32, 37, 40) have significant difference between the He-sea points group and He-Mu points group (P<0.01, P<0.05), No. 9, 16, 31, 33, 35, 38 peaks are the new peaks, No. 8, 15, 18 , 22, 25 peaks are the disappeared peaks. Point out that acupuncture has effect on the serum metabolic products of gastric ulcer mice. During three acupuncture groups, He-Mu points group has the most interrelated metabolic products. So as a result, He-Mu points compared with single points has specific expression, there is a certain synergy.4 There are 3 same metabolic products during the three acupuncture groups separately in serum and urine of gastric ulcer mice, and the correspounding peaks have the same bases with different peak areas. Point out that acupunctured on different points could generate different symbolical metabolic products.Conclusion:1 Acupuncture can effect the gastric injury restoration.2 Stress can influence the metabolite expression of mice's serum and urine. 3 Effects of acupuncture on stress ulcer serum metabolite profiling have specific expression, with new peaks appeared, and the specific expression of acupuncture on He-Mu point better than acuncture on a single point.4 Effects of acupuncture on stress ulcer urine metabolite profiling have specific expression, with new peaks appeared and new peaks disappeared, and the specific expression of acupuncture on He-Mu point better than acuncture on a single point.5 He-Mu points have some specific synergy on serum and urine metabolite profiling of the stress ulcer mice. |
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