Trend Of Apoptosis Accompanying The Aging Of Rats And The Influence Of Swimming In Apoptosis

The hyperplasia, differentiation and death of cell goes through the whole life of multicellular organism. All of which are the same important to life. Early from the 1960s, researchers have noted the two ways of cell "decease", one is necrosis, as all known; the other is apoptosis, with different histological, physiological and biochemical characters. Obviously, necrosis is the "accidental death" of cell caused by nosogenesis; while apoptosis is an ordered "suicide" process controlled by gene that has heredity features, and generally belongs to physiological death process of cell.Aging, associated with death is a normal physiological process that none of the organisms could get rid of. Even if any individual who have no deadly illnesses, will have to face up to such process.Researches have indicated that physical activity is not only the manner of delaying cell aging, but also the inducement of apoptosis. Accompanying aging process, all kinds of cell decline differently. It is important to research the "clear-away" and remodeling procedure of aged or injured cell for aging resistant aspect. It is also important to confirm whether the function of aged or injured cell is cleared away via apoptosis, or the function of the action of aged cell suicide itself to increasing the aging process would be the mechanism of somatic self-remodeling. Moreover, the relationship between cell aging, apoptosis and long term aerobic exercise needs further research.Goal of present studyPresent study was conducted to give evidence to the hypothesis of the existence of the clear-away of injured cell via apoptosis in rats. This was carried out in a chronic swimming model by observing the aging, apoptosis and development characters of peripheral blood leukocyte, hepatocyte and cardiac muscle cell. The other goal of the study was to explain the possible mechanism of the function of aerobic exercise to delay aging process on the cell (molecular) level. This was carried out via the simultaneous observation to the albumen expression of apoptosis-control-gene, and the metabolize level of internal free radical. MethodologySubjects of present study were SD rats of 2-month and 9-10-month in age, separately represents the youth group and before old group. The interventiontreatment was swim without weight loading, exercise regimens were 1-hour /day or 2-hours /day, with 6 times / week or 3 times / week. The whole regimen lasted for totally 14 weeks. The apoptosis ratio of peripheral blood leukocyte was measured through Flow Cytometry, the apoptosis ratio of hepatocyte and cardiac muscle cell was measured through Tissue chip combined with TUNEL staining technique; Bax and Bcl-2 genetic expression in tissue was tested via immune histochemistry technique; also, method of xanthine oxidas was used to test the SOD level in serum and tissue; method of TBA colorimetry was used to test the MDA level in serum and tissue; and method of nitric acid deoxidization was employed to the content of NO in serum and tissue. Conventional statistical methods were used for data analysis. Results1. There were no correlation between the apoptosis of leukocyte and aging. This is especially important to maintain the immunization function by keeping the stable number of short-life cell. Long-term aerobic exercise had an obvious relation to the apoptosis of peripheral blood leukocyte, while the ratio of leukocyte apoptosis had the trend of increasing with exercise workload. Among which, the 2-hours group had a maximum while every-every other day group had a minimum apoptosis ratio. The phenomenon was similar in both groups of youth and before aged. Results implicated that long term aerobic exercise could effectively facilitate the apoptosis of leukocyte in order to ensure the renovate ratio of short-life cells which would be very important to maintain physical immunization function. This might also be one of the cytological mechanisms of how aerobic exercise delays the decline of physical immunization accompanying with aging.2. N...