| ObjectiveSenescence or aging is a natural life course with itself property and law. It is the complex course, which has procedure property and be regulated by heredity. The equilibrium of organism is upset, including a serial material metabolism, information transmission and energy change. The result is a coordination, restriction and influence each other both gene expression and metabolism regulation, which is a natural selection result to adapt itself to circumstances in a long period of time. There are factors to influence aging, including heredity, circumstances, nutrition, exercise and diseases, etc. These factors are used for explaining perplexing life phenomena through combining with the present aging theories. Exercise is a most essential living factor of human being, also a most important circumstance factor of influence aging we know so far. The objectives are discussed on the effects of exercise on the part index related to aging, including basic biochemical data, bone metabolism and apoptosis-associated gene index in the exercise-biochemical and life science, also the mechanism that exercise effects aging in the level of the organisms, organs and genes, respectively. It is beneficial to understanding aging developing and exercise retard aging mechanism in the theory and practice.Methods1. Objects and exercise plans.40 male KM mice of 12 months were randomly divided into 4 groups: the aged control, 10 minute exercise, 20 minute exercise, 40 minute exercise, and 10 mice per each group. 10 male KM mice of 3 months were the young control and 10 male KM mice of 7 months were the adult control. Each exercise group was carried out the exercise plan respectively. The exercise plans included: exercise type was swimming; The swimming time of 10 minute exercise group and 20 minute exercise group were 10 minute and 20 minute each day respectively. The swimming time of 40 minute exercise group were 20 minute in the first and second week, 30 minute in the 3 th and 4th week, 40 minute in the 5th to 12th week. Exercise was carried out for total 12 weeks, 1 time each day, and 5 times each week. The aged control, the young control and the adult control were observed 12, 1, 1 weeks, respectively. Each mouse was anormal nutrition.2. Samples were collected, prepared and measured.The samples of blood were divided into two portions after centrifugation, then measured biochemical data and serum AGEs respectively. Two femur were separated and cleaned, then measured bone mineral density (BMD) and bone biomechanical index respectively. The heart, muscle and liver tissues were collected, and heart tissues were divided into two portions, muscle and liver tissues into three portions respectively. Two samples were measured for AGEs, bax and bcl-2 genes in the level of mRNA respectively. One sample was used for immunohistochemistry after formalin-fixed and made sections.Glu, TG, ALT, ALP and CRE were measured by using semi-automatic biochemical instrument; Bone mineral density and bone biomechanical index were measured by using solid physic density instrument and biomechanical instrument respectively. Serum AGEs were measured using fluorescent method or technique by high-effect liquid chromatographic instrument. The expression levels of bax and bcl-2 mRNA were measured by using immunohistochemistry and RT-PCR.Results1. Effects of exercise on body composition, Glu,TG, ALT, ALP and CRE.Body weight was a slow decreasing as an increasing age in the aged control and all exercise group. The decreasing trend of body weight was slower in each exercise group than that in the aged control. Compared with the young , adult and the aged control, Glu was a significant decrease in each exercise group (p<0.01, p<0.001). Glu was lower in 40 minute exercise group than that in 20 minute exercise group(p<0.01). Compared with the aged control and 10 minute exercise group, TG was a significant decrease in 40 minute exercise group (p<0.05, p<0.01) . There were no significant difference on Glu or TG between the aged control and 10 minute exercise group. Compared...
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