| Objective:The experimental study observes the curative effect of Shen-fu Ling application cooperated with Tripterygium wilfordii poly glucosidlum treat for the refractory nephropathy rat with renal damage; And to investigate the inflammation factors, nephrin and the influence of the lipid metabolism, provides the theory basis for clinical application.Methods:According to the principle of random divided into four groups, for normal group, the model group, the control group (tripterygium wilfordii more filling glucoside stomach), the treatment group (renal apply spirit with external tripterygium wilfordii more filling glucoside stomach). Model group, the control group, the treatment group big rat tail vein one-time injection doxorubicin6.0mg/kg (doxorubicin press2mg/ml soluble in saline, or3.0ml/kg). Normal rats, injection amount saline.7day began to fill the stomach, normal group, the model group stomach saline solution filling every day, dose1ml/100g; The control group stomach tripterygium wilfordii filling every day more glucoside suspension liquid (concentration for2mg/ml) dose1ml/100g; The treatment group in the control group based on treatment in rat god Shu, spleen and kidney que feishu on a topical renal apply spirit. Before and after the experiment began two weeks, four weeks collecting rat urine testing24hours24urine protein quantitative; After collecting the serum albumin detection (ALB), urea nitrogen (BUN), creatinine (Scr), total cholesterol (CHOL) and triglycerides (TG); Western Blot methods detection in kidney tissues across a cell membrane glycoprotein (nephrin), nuclear factor-K B, transformation growth factor-β level; Polymerase chain reaction detection in kidney tissues nephrin; Immunohisto chemical method in detecting kidney tissues apolipoprotein A-I (apoA-I) and decorin protein level.Results:1. The experiment day14, the28days, the model group, the control group and the treatment group was significantly lower than the normal rats weight group (P<0.01), model rats, weight and the treatment group was significantly lower than the control group (P<0.01), and the control group and the treatment group, weight is no obvious difference (P>0.05);2. The experiment day14and28days, model group, the control group and the treatment rats,24h urine protein was significantly higher than normal group; And model group, compared to the control group and the treatment group24h urine protein quantity is restrained significantly (P<0.01); The treatment group24h urine protein28days of lower than that in control group (P<0.01);After the end of experiment, compared with normal group, model group, the control group, the treatment group serum albumin leels significantly lower P<0.01); And model group, compared to the control group and the treatment group increased significantly (P<0.01); Treatment group was obviously higher than those in the control group (P<0.01);After the end of experiment, compared with normal group, model group, the control group, the treatment group of serum cholesterol, triglyceride levels increased significantly (P<0.01); And model group, compared to the control group and the treatment group reduced significantly (P<0.01); Treatment group was obviously lower than that in control group (P<0.01);Serum urea nitrogen and muscle groups with no significant differences between;By light microscopy found normal rats, mesangial area without hyperplasia, sediment, basement membrane has not seen the pathological changes, capillary open good; Model rats, visible diffuse mesangial area broadens, and film cells, department of membrane matrix hyperplasia, capillary lumen; Tripterygium wilfordii much glucoside group and kidney apply spirit merger tripterygium wilfordii much glucoside in different degree of lesions reduce.By electron microscopy, normal rats, glomerular basement membrane complete structure, the film has not seen the hyperplasia, clear and complete foot processes; Model group glomerular podocyte foot processes of widely fusion, the film substrate increased, thickening of glomerular basement membrane, the membrane cells and basement membrane not seen in obvious things electron dense deposits, the glomerular capillary open, endothelial cells mild swelling, etc. Tripterygium wilfordii much glucoside group and kidney apply spirit merger tripterygium wilfordii much glucoside in model group pathological changes significantly reduce group, the foot processes of fusion, the film substrate mild increase, glomerular basement membrane thickening ease.3. The experiment ends, compared with normal group, model group, the control group, the treatment group rats in the kidney tissues nf-kappa B, TGF-obviously up-regulated (P<0.01); And model group, compared to the control group and the treatment group reduced significantly (P<0.01); Among them the nf-kappa B level of treatment group is obviously lower than those of the control group (P<0.01);4. The experiment ends, compared with normal group, model group, the control group, the treatment group rats in the kidney tissues nephrin protein expression obviously down-regulated (P<0.01); And model group, compared to the control group and the treatment group increased significantly (P<0.01); Treatment group was obviously higher than those in the control group (P<0.01);5. The experiment ends, compared with normal group, model group, the control group, the treatment group rats in the kidney tissues apoA-I level express significantly; And model group, compared to the control group and the treatment group increased significantly (P<0.01); Compared with the normal group, model group, the control group, the treatment group rats in the kidney tissues decorin express increased significantly (P<0.01); And model group, compared to the control group and the treatment group reduced significantly (P<0.01); Treatment group was obviously lower than in control group (P<0.01);Conclusion:1. Kidney apply spirit external merger tripterygium wilfordii more persistent nephrotic syndrome in improving glucoside rat nutrition, reduce albuminuria and serum cholesterol and triglycerides, and increase serum albumin is good effect.Kidney apply spirit with external tripterygium wilfordii treatment plant more rats after kidney pathological change significantly reduce, apply for kidney spirit treatment refractory nephrosis rats, reduce the kidney damage provides histopathological on the basis. 2. Kidney apply spirit with external tripterygium wilfordii much glucoside can inhibit the nf-kappa B, TGF-β expression, reduce the persistent nephrotic syndrome rats of glomerular inflammation and damage to the conservation restraining glomerulus.3. Kidney apply spirit with external tripterygium wilfordii much glucoside can through regulation foot cell and molecular nephrin protein, thus protecting glomerular stage of the diaphragm of the structure and function of integrity, to reduce refractory nephrosis rats proteinuria.4. This study proves renal apply spirit with external tripterygium wilfordii much glucoside can can improve apoA-I, decorin expression, reflected in the improvement of lipid metabolism of the good function to protect the persistent nephrotic syndrome rats kidney from damage. |
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