| Purposes To establish rat Chronic nonbacterial prostatitis model using the17beta estradiol combined castration and to evaluate histo-pathological changes of prostate tissues.Methods Eighty2months SPF male Sprague-Dawley (SD) rats were randomly divided into8groups,namely group A:blank control group (n=10);group B:saline group (n=10);C sham operation group (n=10); group D:castration group (n=10); group E:17-beta Estradiol (17beta Estradiol)(0.25mg/ml/kg)2(n=10);group F:17-beta Estradiol (0.15mg/ml/kg)(low dose group) combined Castration group (n=10); group G:17-beta Estradiol (0.20mg/ml/kg)(middle dose group) combined Castration; group H group (n=10):17-beta Estradiol (0.25mg/ml/kg)(high dose group) combined Castration (n=10). To evaluate the pathological changes in different groups according to the international prostatitis histological diagnosis.Results The pathologic appearance of the prostate tissue of the different groups were observed with HE staining and microscope. Hyperemia and edema of rat prostate and adhesive surrounding tissues were observed by naked eyes in group H.Structure damage accompanied by uneven lymphoid tissue hyperplasia, prostate gland duct damage or partial basement membrane damage with microscope.Inflammatory cells were distributed in groups, more visible lymph nodules or follicle formation could be observed also, group D and group E, group F and group G then were characterized by mild or moderate inflammation with visible scattered lymphocytes and mononuclear cells infiltration. Gross morphology of group A and group B, group C rats prostate showed complete capsule,smooth surface.There is no obvious tissue inflammation. Baed on evaluation of core indicators of inflammation in different groups. Compared with group D, group E, group F and group G,there is statistically significant in group H fo rat histological inflammation grading of chronic prostatitis (P<0.05). Medium or severe inflammation is observed in group H. However, compared with group D, group E, group F and group G,there is statistically significant in group H in terms of inflammation scope(P<0.05). Focal or multifocal performance of inflammation was observed in group D, group E, group F and group G. Multifocal or diffuse inflammation was observed in group H.Conclusions17beta estradiol combined castration can be induce to establish SD rat chronic nonbacterial prostatitis (CNBP) model.Histopathological manifestations are similar to clinical histopathological changes. There is positive correlation between histopathological inflammation and17beta estradiol concentration.Single17beta estradiol or castration rats can induce rats prostate mild, focal inflammation,but it is not close to clinical manifestations. Background Prostatitis, especially Chronic Prostatitis has been main problem for male.However,the pathogenes of Chronic Prostatitis remains unclear. For its high incidence and high recurrence rate, Chronic Prostatitis affects peoples’ health seriously.It can lead to sexual dysfunction, male infertility.Therefore, it has an impact on physical and mental health, quality of life and family happiness. So,to clear the pathogenesis of chronic prostatitis is crucial.Current etiological research of CP have been focused on pathogenic microorganism, urine reflux, abnormal function of epithelial cells, neuroendocrine abnormalities, etc.In recent years, related studies have shown that there is a potential relation between neuroendocrine and CP.Prostate Neuroendocrine cells (Neuroendocrine cell, NEC),are also called the Amine Precursor Uptake Decarboxylation And (APUD) cells. Studied found that Neuroendocrine cell exist between secretory epithelial cells.These cells can secrete hormone polypeptide and serotonin, which regulate cell growth and secretion of prostate. Through the adoption of17beta estradiol combined castration surgery (bilateral testes excision),We establish SD rat chronic prostatitis model.These important landmark neuroendocrine cells:Chromogranin A(CgA), Neuronal specific enolase(NSE), Nerve growth factor(NGF) and substance P are explored in order to understand role of neuroendocrine differentiation in chronic prostatitis.Methods We used SD rats to establish animal models of chronic nonbacterial prostatitis, investigated important marker proteins of rats neuroendocrine cells in different group. These makers included chromogranin A (CgA), neuron specific enolase (NSE), nerve growth factor (NGF) and substance P (SP). Expression level protein and gene of each group were probed by immunohistochemical(IHC), western blot(WB), enzyme-linked immunosorbent assay(ELLSA), and real-time quantitative PC(qPCR). Correlations between chromogranin A(CgA) and substance P(SP), neuron specific enolase(NSE) and substance P(SP), nerve growth factor(NGF) and substance P(SP) were analyzed, respectively.Results1. Immunohistochemistry showed that expression of CgA、NSE、NGF、SP in model group H was higher than group A、roup B、group C、group D、group E respectively.2. Western blot qualitative study showed that expression of CgA, the NSE, NGF in chronic prostatitis model group (group H) was higher than the other groups (P<0.05)..3. Quantitative enzyme-linked immunosorbent results showed that serum content of CgA、NSE、NGF and SP in rats model group (group H) content was higher than other groups (P<0.05).Correlation analysis of CgA and SP expression have positive correlation (gamma=0.35, P<0.01); NSE and SP (gamma=0.29, P<0.01); NGF and SP (gamma=0.50, P<0.01).4. Real-time quantitative PCR study found that compared with other groups,CgA (F=109.4)、NSE (F=68.4)、NGF(F=65.4),SP(F=61.1) expression was higher in chronic prostatitis model group (P<0.05).Correlation analysis of CgA and SP showed positive correlation (gamma=0.45, P<0.01); NSE and SP (gamma=0.53, P<0.01); NGF and SP (gamma=0.37, P<0.37).Conclusions1. Expression of CgA, NSE, NGF were increased in17-beta estradiol subcutaneous injection combined castration CAP rat model, which prompt the neuroendocrine differentiation.We speculatea that up-regulation is induced by17beta estradiol and (or) castration.2. CgA and SP、CgA and NSE、CgA and NGF、CgA and SP showed positive correlation respectively, which indicated that neuroendocrine differentiation might be associated with inflammation. As SP is an important factor causing pain. the neuroendocrine cells may participate in mechanism of chronic prostatitis neurogenic pain. |
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