| Objective:1.Observe the effect on changes of blood lipid metabolic and organization form and the influence of associated serum inflammatory factors of atherosclerosis(AS) rabbit by cake-separated moxibustion.2.Observe the effects on expression of PPARγ、SR-B1 in SR-B1 mediated pathway of reverse cholesterol transport(RCT) of AS rabbit by cake-separated moxibustion,explore the mechanism of action of anti-AS by cake-separated moxibustion.Methods:The 40 healthy purebred male New Zealand rabbits divided into fivegroups randomly: blank control group,model group, cake-separated moxibustion group,direct moxibustion group and simvastatin group. HLP combined AS rabbits were established by simple high-fat feeding method and given different intervention treatment.Then, the blood samples were collected after ear marginal vein anesthesia. The aorta and liver samples were taked from the death rabbits and observed by HE staining. The levels of total cholesterol(TC),triglyceride(TG),low-density ipoprotcin(LDL), high-density lipoprotein(HDL)in serum were measured by enzymic method.The levels of tumor necrosis factor-α(TNF-a),interferon-γ(IFN-γ), interleukin-6/10(IL-6,IL-10) inserum were measured by enzyme-linked immune sorbent assay(ELISA).The expressions of peroxisome proliferator-activated receptor-γ(PPARγ) and scavenger receptor B1(SR-B1) in liver were measured by western blot(WB). Theexpressionsof mRNA of PPARγ and SR-B1 in liver were measured by real-time quantitative polymerase chain reaction(real-time PCR).Result:1.The surface of aortic vascular wall inblankcontrolgroup was smooth, the layers of the structure was clear, endothelial cells was integrated and continuous, smooth muscle cells were aligned and orderly without the deposition of lipid and plaque.Aortic vascular wall in model group was obviously thickened, falls off within the cortex, the wall was full of lipid and plaque, a large number of foam cells appeared.The intima was thickened slightly and of the smooth muscle cells were uniform thickness and neat rows with few foam cells in cake-separated moxibustion group and direct moxibustion group.In simvastatin group,the intima was thickened slightly lining slightly thickened with foam cell formation, each layer structure was integrated compared with model group,, the intima had a small amount of lipid, the obvious plaques were not found.The structure of liver tissue and hepatic cell nucleus were aligned, hepatic sinus without blood stasis were empty, hepatic cell were neat and uniform cytoplasm had no lipid degeneration.In model group the structure of liver tissue was not clear, some hepatic sinus were narrow or missed, endothelial cells were not obvious, liver cells with transparent cytoplasm were swelling, part liver cells with fatty degeneration had lipid drops, fat cavitation diffused cloth, infiltration of lymphocytes and plasma cells occurred in interstitial tissue;Incake-separated moxibustion group and direct moxibustion group liver tissue structure was obvious, depositionof lipid drops were reduced in liver cells with fatty degeneration slightly and mild inflammatory reaction.In simvastatin group,liver fatty the degree of liver cells with degeneration was more mild compared with model group, few cells had a few lipid droplets cavity and hydropic degeneration,liver tissue structure were changed and the nucleu position of most liver cells hadn’t changed.2.TC、TG、LDL-C in serum in model group were significantly higher than those in blank control group,with statistical significance(P<0.01),HDL-C in serum in model group were significantly lower with statistical significance(P<0.01).In cake-separated moxibustion group, direct moxibustion group and simvastatin group,TC、TG、LDL-C in serum were significantly lower and HDL-C in serum were higher than those in model group, with statistical significance(P<0.01). The cholesterol content of the aorta, liver tissue and adipose tissue in model group were significantly higher than those in blank control group,with statistical significance(P<0.01). In cake-separated moxibustion group,direct moxibustion group and simvastatin group, the cholesterol content of the aorta, liver tissue and adipose tissue were significantly lower than those in mode group,with statistical significance(P<0.01).3.IL-6、TNF-α、IFNγ in serum in model group, direct moxibustion group and simvastatin groupwere significantly higher than those in blank control group,with statistical significance(P<0.01 or P<0.05),in cake-separated moxibustion group, IL-6、IFNγ in serum was without statistical significance and TNF-α in serum was significantly higher than those in blank control group with statistical significance(P<0.05).In cake-separated moxibustion group,direct moxibustion group and simvastatin group,IL-6、TNF-α、IFNγ in serum were significantly lower than those in model group, with statistical significance(P<0.01 or P<0.05). In model group and simvastatin group,IL-10 in serum were significantly lower than those in blank control group,with statistical significance(P<0.05),IL-10 in serum in cake-separated moxibustion group,direct moxibustion group and simvastatin group were significantly higher than those in model group,with statistical significance(P<0.01 or P<0.05)4.Compared with blank control group, the protein expression of PPARγ and SR- B1 in liver in model group were significantly lower,with statistical significance(P<0.01 or P<0.05), the protein expression of PPARγ and SR-B1 in liver in cake-separat-ed moxibustion group were significantly higher, with statistical significance(P<0.01 or P<0.05), the proteinexpression of PPARγ in liver in direct moxibustion group were significantly higher, withstatistical significance(P<0.01 or P<0.05), the protein expression of SR-B1 in liver in direct moxibustion group was without statistical significance. Comparedwithmodelgroup, the protein expression of PPARγand SR-B1 in liver in cake-separatedmoxibustiongroup, direct moxibustion group and simvastatin group were significantly higher, with statistical significance(P<0.01 or P< 0.05). Compared with cake-separated moxibustion group, the protein expressionof PPARγ and SR-B1 in liver indirectmoxibus-tiongroup and simvastatin group weresignificantly lower with statistical significance(P<0.01 or P<0.05).Compared with blank control group, the mRNA expression of PPARγ and SR-B1 in liver in model group were significantly lower, with statistical significance(P<0.01 or P<0.05), the mRNA expression of PPARγ and SR-B1 in liver in cake-separated moxibustion group and direct moxibustion group were significantly higher, with statistical significance(P<0.01 or P<0.05), the mRNA expression of PPARγ in liver in simvastatin group were significantly higher, withstatistical significance(P<0.01 or P<0.05), the mRNA expression of SR-B1 in liver in simvastatin group was without statistical significance. Compared with model group, the mRNA expressionof PPARγ and SR-B1 in liver in cake-separated moxibustion group,directmoxibus-tiongroup and simvastatin group were significantly higher, withstatistical signify- cance(P<0.01 or P<0.05), there was no statistically significant difference between three groups. Compared with cake-separated moxibustion group, the mRNA expressionof PPARγ and SR-B1 in liver indirectmoxibus-tiongroup and simvastatin group were significantly lowerwith statistical significance(P<0.01 or P<0.05).Conclusion:1.Cake-separated moxibustion can adjust benignly to blood lipid metabolic changes and repair the pathological changes of arterial intima and liver tissue of AS rabbit.2.The effect of cake-separated moxibustion resisting atherosclerosis may be related to the cake-separated moxibustion’s influence on inflammatory factors of atherosclerosis rabbit serum.3. Cake-separated moxibustion can increase the protein and mRNA expression of PPARγ and SR-B1 in liver in SR-B1 mediated pathway of RCT, activate PPARγ, induce the expression of SR- B1.It can promote RCT and inhibit the formation of AS and has an effect on anti-AS. It maybe one of the mechanisms of anti-AS by cake-separated moxibustion.... |
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