| Objective: To observe the effect of electroacupuncture at the meridian acupoints on the expression of mi C137/EZH2 and BDNF in the brain tissue of rats with cerebral ischemic injury.To evaluate the effect of acupuncture and moxibustion on cerebral ischemic injury Damage is related to its promotion of neuronal proliferation and maturation,while Me CP2 phosphorylation plays a role in promoting it.It also provides theoretical support and experimental basis for the treatment of brain.Methods: The model of MCAO cerebral ischemia was established by external carotid artery occlusion.The model of Me CP2 in cerebral ischemia was detected by immunohisto chemistry.The expression of Me CP2 and BDNF in cerebral ischemia was compared with that of model group The expression of Me CP2 m RNA,mi R137 / EZH2 and BDNF m RNA in cerebral ischemia were detected by Q-PCR.Western-Bloting was used to detect the expression of Me CP2,p Me CP2.Results: 1?The neural function defect scale: with the model group,the heart meridian group,the pericardial cavity by the group in the 7 d,14 d and 21 d scores were lower(P < 0.05,P < 0.01),two groups can be improved in different degrees of MCAO/R rats nerve defect scale,effect of the two groups(P>0.05).Is: in the group and model group: with 1 d ratio,21 d scores significantly decreased(P<0.05),and rats have self-healing function.Heart meridian,the pericardium cavity via group: over time,each phase point score were decreased(P<0.05,P<0.01),suggesting the curative heart sutra pericardium meridian group to improve the effect of nerve function effect of rendering time.2?Me CP2 m RNA,protein: compared with normal and pseudosurgical group,the model group,the heart meridian group,and the pericardium group were raised in Me CP2 m RNA and protein expression in 7d,14 d,21d(P<0.01).Compared with the model group: the Me CP2 m RNA(P<0.01)of the meridium(14d,21d),pericardium(7d-21d),and the protein(P<0.01).The pericardial meridium was lower than the pericardial group(P<0.05)in 21 d.Group comparison: model group Me CP2 m RNA,protein expression: 7d,14 d >21d>1d(P <0.05);Heart meridian,pericardial meridian group: 7d > 14 d > 21d(P<0.05,P<0.01).In the case of cerebral ischemia injury,the MCAO/R rat body was in the 7d-21 d,which was called Me CP2 m RNA and protein expression,and over time,the expression of the expression decreased gradually.The two meridien groups were able to promote Me CP2 m RNA and protein expression in the 7-21 d,and then increased the effect of the rat attenuation of Me CP2 m RNA and protein expression.And weakened its role,the pericardium meridian group(7d)before the heart meridian(21d),at the time of 21 d pericardium with drop effect is superior to the heart sutra,and has a certain timeliness and meridian specificity.3?p Me CP2:The expression of p Me CP2 in 7d,14 d,21d and p Me CP2 were raised in the group,the group of the normal group and the pseudosurgical group.The p Me CP2 expression of the meridium and pericardium in 7d,14 d,and 21 d is more than the model group(P < 0.01),and the two effects are quite similar.Model group,heart meridian group,pericardial meridian group: 7d > 14 d,21d > 1d(P<0.05,P<0.01).After suggesting cerebral ischemia injury,the MCAO/R rat body promoted p Me CP2 in 7-21 d,which decreased in 14-21 d over time.The two meridions were able to improve the expression of p Me CP2 by varying degrees from 7-21 d,while delaying the effect of p Me CP2 expression on 7-21 d.And the two caves are equivalent.4?mi R137: with the normal group and control group,model group(7 d,14 d),heart meridian group(7 d,14 d and 21 d),the pericardium meridian group(7 d,14 d and 21 d)were increased(P < 0.05,P < 0.01);The expression of the meridium and pericardial meridium in 7d,14 d,and 21 d is higher than the model group(P<0.01).The pericardial meridium was expressed in 7d and 14 d above the meridium group(P < 0.05).Group comparison: model group: 7d,14 d >21d,1d(P<0.05,P<0.01)Heart meridian: 7d > 14 d > 21d(P< 0.05,P<0.01);Group group: 7d > 14 d >,1d(P < 0.05,P<0.01).After suggesting brain ischemia injury,the MCAO/R rats were able to express mi R137 in 7d-21 d,and this effect decreased over time.However,the acupuncture point group(7d,14 d,21d)and the pericardium group(7d,14d),and the pericardium group(7d,14d),were obvious.It is shown that the pericardial meridian can effectively promote the expression of mi R137,and the pericardium is superior to the heart(7d,14d).5?Ezh2 m RNA: the Ezh2 m RNA expression of the model group 21d(P < 0.05),compared to normal and pseudosurgical group.The expression of Ezh2 m RNA expression of the heart meridian group,the pericardium group,the pericardium group,and the Ezh2 m RNA in the pericardium group,the pericardium group,the pericardium group,and the Ezh2 m RNA expression of the normal group,the pseudooperative group,and the model group were decreased in different degrees(P < 0.05,P < 0.01).Comparison of the heart and hole groups: the degree of expression of Ezh2 m RNA in the pericardium was comparable to the meridium group(P > 0.05).Group comparison: the model group: Ezh2 m RNA expressed at each point of time(P > 0.05).Heart meridian group: 1d > 14 d,21d(P < 0.05);Pericardium group: 1d,7d > 14 d,21d(P < 0.05,P < 0.01).After suggesting cerebral ischemia injury,the MCAO/R rat body promoted Ezh2 m RNA in 21 d.And cupping heart sutra pericardium via(14 d and 21 d)can inhibit in different degrees Ezh2 m RNA expression,show the curative heart sutra pericardium meridian can have inhibition of Ezh2 m RNA expression function,and the role of the two classics.Conclusion: 1.After cerebral ischemia injury,the body produces a stress response,which is called Me CP2 phosphorylation,which decreases after the peak of 7-21 days,while also promoting the expression of mi R137.2.EA the heart meridian and the Heart pericardium meridian can promote Me CP2 phosphorylation,the expression of mi R137,and inhibit Ezh2 m RNA expression.3.Cupping heart sutra,the pericardium meridian acting time and its effect on promoting nerve repair in sustainability have certain meridian specificity. |
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