| Objective:To observe the effect on protein expression of Caspase-3,TNF-a and IL-1βin ischemic penumbra of cerebral ischemia reperfusion rats treated by acupuncturing DU meridian;and to explore the mechanisms of acupuncturing DU meridian in protecting ischemia reperfusion tissue.Methods:Focal cerebral ischemia rats model were used for the object of research,and they were treated separately by acupuncturing DU meridian acupoints and Yangming meridian acupoints.Then the changes of neurobehavioral scores were observed.Besides,the changes of the expression of protein Caspase-3,TNF-a and IL-1βwere detected by Western blot method before and after the treatment.Results:(1)neurological score:Both the DU meridian acupoints acupuncture and the Yangming meridian acupuncture can significantly improve the neurological score before and after treatment compared with the model group(P<0.05), and the DU meridian acupoints acupuncture group has the better effect than the Yangming meridian acupuncture group(P<0.05). (2)Protein expression of Caspase-3,TNF-αand IL-1β:Caspase-3,TNF-αand IL-1βin the brain tissue of the sham operation rats are on the level expression;and they are all significantly higher expressed in the model group than in the normal group(P<0.01).In the DU meridian acupoints acupuncture and the Yangming meridian acupuncture group,all the three protein expressions are lower than the model group(P<0.01&P<0.05),but higher than the sham operation and the normal,and the DU meridian acupoints acupuncture group has a more significant effect.(P<0.05).Conclusion:Acupuncturing DU meridian acupoints can more significantly improve the neurological damage after ischemia-reperfusion injury of rats,and has more significantly effect on reducing the protein expression of Caspase-3,TNF-a and IL-1βthan acupuncturing the Yangming meridian to achieve the purpose of anti-ischemic brain injury.This maybe the mechanisms of acupuncture protecting the injury brain. |
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