| This thesis consists of two parts. Part one is review. Part two is research reports.Molecular imprinting is an effective technique that prepared the polymer, which have selective recognition function. Its principles, preparation, development in the recent years and its applications in analytical chemistry are reviewed in the first part. 1.Molecular imprinting -chemiluminescence analysisChemiluminescence (CL) analysis has been frequently used to the analysis of a wide variety of important inorganic and organic compounds due to its numerous advantages such as low detection limit and wide linear dynamic range, which both can generally be achieved with simple and relatively inexpensive instrumentation. Unfortunately, the relatively low selectivity of the CL method itself limits its direct application to the analysis of analyte in complicated sample. Great efforts were made to overcome this shortcoming and the most common way is to use a separation technique, such as liquid chromatography and capillary electrophoresis, prior to CL detection. Most of these methods not only required relatively complicated and expensive instruments, but also suffered from the incompatibility of separation conditions and CL detection conditions in many applications.Molecular imprinted polymer has been known as a new synthetic material capable of molecular recognition. The attractive features of MIP, such as mechanical strength, ease of preparation and stability in harsh environments allow it to be used in many fields. Now combining the selectivity of the molecular imprinting technique with the sensitivity of CL is a good idea to solve the problem.(1) Flow injection chemiluminescence determination of epinephrine using epinephrine-imprinted polymer as recognition materialA flow injection chemiluminescence method using epinephrine-imprinted polymer as recognition material was investigated for the determination of epinephrine.The analyte, epinephrine, was selectively and temporarily adsorbed on the epinephrine-imprinted polymer, which was packed into a flow cell placed in front of the window of photomultiplier tube. Afterward, the chemiluminescence reagent, thecombining stream of luminol (presence of potassium ferrocyanide) and potassium ferricyanide flowed through the flow cell and reacted with epinephrine adsorbed on the polymer to produce strong chemiluminescence. The chemiluminescence intensity responded linearly to the logarithm of the concentration of epinephrine within 5.0+0-9-1.0+10-7mol/L range with a detection limit of 3+10-9mol/L. The relative standard deviation for 2.0+10-8mol/L of epinephrine solution was less than 5% (n=7). Interference experiments demonstrated that the application of molecular imprinted polymer to chemiluminescence analysis could improve the selectivity of method greatly. Tests of the recovery for known amount of epinephrine in serum were carried out and satisfactory results were obtained. At the same time, the binding characteristic of the polymer to epinephrine was evaluated by batch method and dynamic method.(2) Flow-through chemiluminescence sensing system for paracetamol based onmolecularly imprinted polymerA new flow-through chemiluminescence sensing system using mole-cularly imprinted polymer as molecular recognition material was developed for the determination of paracetamol. The method is based on the inhibitory effect of paracetamol, which was selectively adsorbed in the polymer imprinted by paracetamol, on the chemiluminescence reaction of luminol with potassium ferricyanide in alkaline medium. The decreased CL intensity responded linearly with the logarithm of the concentration of paracetamol over 7-100mol/L range. The detection limit for paracetamol was 3mol/L and the relative standard deviation was less than 5% (50.0 mol/L paracetamol solution, n=9). The method was successfully applied to the analysis of paracetamol in the diluted urine samples.2.The study of flow injection chemiluminescence determination ofchlorpheniramineIt is found that the oxidati...
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