Application Of Molecular Imprinting-chemiluminescence Analysis In Pharmaceutical Assay

This thesis includes a review and a research section. The review includes the preparation, characterization of molecular imprinting polymer (MIP), the recognition mechanism of MIP and development of this field in the future. The research section consists of three parts. In the research section, we have synthesized MIPs of norfloxin, Metamizde Sodium and phenacetin, and developed flow chemiluminescence-molecular imprinting analysis using these three imprinted polymers as recognition material.It is well known that chemiluminescence(CL) method is an important detection method, with the advantages of low detection limit, wide linear dynamic range and inexpensive instruments. However, relatively poor selectivity of CL method limits its application to complicated samples. So, it is urgent and significant to improve the selectivity of CL method.Molecular imprinted polymer (MIP) is an important molecular recognition material appeared in recent years. Recognition ability of some MIP to target molecule was comparable with crude molecular recognition system, such as enzyme to matrix, antibody to antigen, receptor to hormone. Currently, MIP has been successfully applied in chiral separation, solid phase extraction, chromatography separation, sensors and immunoassay etc.The research section includes three parts. In Part one, a selective flow chemiluminescence method was developed for the determination of norfloxacin, using norfloxacin-imprinted polymer as recognition material in cerium(IV)-Na2SO3-norfloxacin CL method. The chemiluminescence intensity was responded linearly to the concentration of norfloxacin within 1.0×10-7~1.0×10-5mol/L with a detection limit of 3×10-8mol/L. The relative standard deviation for 5.0×10-7mol/L of norfloxacin was 2.4% (n=7). This method was applied to determine norfloxacin in urine sample and satisfactory results were obtained.In part two, we found Mn (IV)- formaldehyde- metamizde sodium CL system, and synthesized metamizde sodium-imprinted polymer using methacrylic acid(MAA) as functional monomer and ethylene glycol dimethacrylate (EGDMA) as cross-linker, then we applied this metamizde sodium-imprinted polymer to Mn(IV)-formaldehyde-metamizde sodium CL system, and established a flow chemiluminescence-molecular imprinting analysis for the determination of metamizde sodium in complicated samples. The chemiluminescence intensity was responded linearly to the concentration of metamizde sodium within 1.0×10-7~1.0×10-5mol/L with a detection limit of 3×10-8mol/L. The relative standard deviation for 1.0×10-6mol/L of metamizde sodium was 3.4% (n=7). The flow injection chemiluminescence method to determine, based on enhancing the chemiluminescence from the oxidation of metamizde sodium by soluble, has been developed.In part three, we established the flow injection chemiluminescence method of determining phenacetin with KMnO4-formaldehyde-phenacetin system, then using methacrylic acid (MAA) as functional monomer and ethylene glycol dimethacrylate (EGDMA) as cross-linker, synthesized phenacetin-imprinted polymer and developed flow chemiluminescence-molecular imprinting analysis for the determination of phenacetin. The chemiluminescence intensity was responded linearly to the concentration of phenacetin within 1.0×10-6~1.0×10-4g/mL with a detection limit of3×10-7 g/mL. The relative standard deviation for 5.0×10-6g/mL of phenacetin was 2.8% (n=7). The method has been applied to the determination of metamizde sodium in Amidopyrini et Paracetamdi Compositae samples and the results were in good agreement with those obtained by pharmacopoeia method.