Studies On Extraction And Purification Of Grape Pigment

The research used grape wine pomace which was squeezed in XinJiang winery as raw material to study extraction efficiency of grape pigment by solvent extraction,microwave irradiation extraction and Compared each way of extraction,determined the optimum solvent extracting and technological conditions,the optimum conditions were beating extraction temperature 80℃,concentration of HCL 1.2%, hot beating extraction time 30min ,concentration of alcohol 60% and the optimum microwave irradiation conditions were microwave power 60% ,concentration of HCL 0.5%,hot beating extraction time 10s,concentration of alcohol 40%,by studying the method of removing solvent in grape pigment oleoresin, efficiency of removing solvent was determined by gas chromatography(GC),we determined vacuum conditions were the safety and efficiency method of removing solvent. By studying the way of purification of grape pigment,determined the optimum mebendazole of paper chromatography,and regarded combining with D152 negtive Ion exchange resin and paper chromatography BAW1(4:1:5)as ideal method got purification grape pigment.In this study,a qualitative analysis of pure pigment in graper:UV-visible obsorption were used, eaction with Alcl3 ,identification of carbohydrate components,partial and complete hydrolysis of pigments calculating A440/Amax ratio and so on. determined pure pigment of grape is peonidin-3-glucoside(Pn-3-glu).and studied the stability of crude grape pigment and pure grape pigment in different conditions,The results shows that the color of pigment varys with Ph , Ph>9 the red color disappears ,iradiation,oxidizing agents,reducing agents,Fe3+in ten kinds of metal positive Ion and CrO42-in eight kinds of negtive Ion all damage to the crude grape pigment and pure grape pigment ,but heat ,microwave and preservatives have better effect on the stability of the crude grape pigment providing reference and evidence for protecting tincture and safety store of grape pigment product.