The Comparison Of Fermentation, Co-fementation And Polysaccharides Activities Of Marasmius Androsaceus And Cordyceps Militaris

Medicinal fungi is a kind of medication which has no toxicity to human. Fungi polysaccharide is one of the major bioactive compounds in medicinal fungi, and has obvious effects on antioxidative and strengthening immune function. On account of lacking of wild resources of medicinal fungi and hard to cultivation, the deep liquid fermentation will have a widest potential application in produce bioactive compounds from fungi. The co-fermentation was provided with the quality of multiple strain accretion, enzyme system mutual complementarity, simplified equipment. The liquid fermentation techniques of medicinal fungi has been paid more attention, research of co-fermentation, however, was rarely reported. This paper is studied about co-fermentation of Marasmius androsaceus and Cordyceps militaris and the biologic active of co-fermentation polysaccharides, including comparison of fermentation characteristic, extraction, isolation and Antioxidant active.The single factor tests and orthogonal experimental designs were applied to optimize the media of M-C Co-fermentation and to optimize the culture condition of Marasmius androsaceus, Cordyceps militaris, M-C Co-fermentation. The results showed that the best fermentation media should be: maltose 3%, peptone 3%, MgSO4 0.2%, KH2PO4 0.3%. The optimum culture temperature, rotation rate, culture time of Marasmius androsaceus were 24℃, 125rpm, 3d, Cordyceps militaris were 25℃, 125rpm, 5d, M-C Co-fermentation were 25℃, 125rpm, 3d, respectively. Under the optimum submerged culture condition, the biomass of Marasmius androsaceus, Cordyceps militaris, M-C co-fermentation were up to 9.30 g/L, 11.90 g/L, 12.76g/L separately, and the crude exopolysaccharide of Marasmius androsaceus, Cordyceps militaris, M-C co-fermentation were up to2.653 g/L, 3.565g/L, 3.123g/L separately.The extraction process of endopolysaccharides from Marasmius androsaceus was optimized by the single factor tests and central composite design. The optimum conditions for extraction process were as follows: extracting temperature was 78℃, extraction time was 1.5h, the ratio of water to sample was 36:1; extracting times was 1.Under the optimum extraction process conditions, the maximum yields of endopolysaccharides were up to 11.59%. The extraction process of endopolysaccharides from Cordyceps militaris was optimized by the single factor tests and orthogonal experimental designs. The optimum conditions for extraction process were as follows: extracting temperature was 70℃, extraction time was 2h, the ratio of water to sample was 30:1; extracting times was 1. Under the optimum extraction process conditions, the maximum yields of endopolysaccharides were up to 21.61 % . The extraction process of endopolysaccharides from M-C Co-fermentation was optimized by the single factor tests and orthogonal experimental designs. The optimum conditions for extraction process were as follows: extracting temperature was 70℃, extraction time was 1.5h, the ratio of water to sample was 20:1; extracting times was 1. Under the optimum extraction conditions, the maximum yields of endopolysaccharides were up to 21.97%.Study on the antioxidative activity of exopolysaccharides and endopolysaccharides from Marasmius androsaceus, Cordyceps militaris, M-C Co-fermentation and M+C mixed. Four kinds of evaluation systems for antioxidative activity were adopted to test their efficiency to scavenge radicals, including hydroxyl free radical·OH, superoxide redical O2÷, DPPH·free radical and anti-lipid peroxidation. The results showed that the ability of thesepolysaccharides and endopolysaccharides to scavenging O2÷were lowly. In the hydroxyl radical scavenging test, the exopolysaccharides EC50 of Marasmius androsaceus, Cordyceps militaris, M-C Co-fermentation and M+C mixed were 0.157 mg/ml, 0.277 mg/ml, 0.1432mg/ml, 0.2203mg/ml respectively and the endopolysaccharides EC50 of Marasmius androsaceus, Cordyceps militaris, M-C Co-fermentation and M+C mixed were 1.024 mg/ml, 0.34mg/ml, 0.00924 mg/ml, 1.3695 mg/ml respectively. The eliminate rate of these polysaccharides can reach to 85%。The eliminate rate of exopolysaccharides and endopolysaccharides of M-C Co-fermentation showed higher and different from others, which was used as a proof for the cooperation and predominance of Co-fermentation. The EC50 of from M-C Co-fermentation was 100 times to others. In the DPPH test, M-C Co-fermentation polysaccharides scavenge DPPH activity were higher than others. The EC50 of endopolysaccharides form M-C Co-fermentation was 2.13 mg/ml. The results of anti-lipid peroxidation showed that these polysaccharides all possess strong activity.