Study On Extraction And Purification Technology Of Polysaccharide From Zizyphus Jujube Mill

In this study, the polysaccharide and protein in zizyphus jujube mill was used as the object, In the purification process, the polysaccharide was study on extracting discolor dialysis and de-protein for further study, the dissoluble protein was extracted dialysis electrophoresis high Performance Liquid Chromatograph, in order to offer theoretical foundation for produce and relevant research. The result is as follows:1.The optimal conditions for hot water extraction was 1:16 of the ratio of sample to water,240 mins of extraction at 100℃and one time, The rate was 5.94%;The optimal conditions for enzyme extraction was pH4.5,60 mins of extraction at 60℃and 0.03% concentration of enzyme, The rate was 7.82%;The optimal conditions for alkali extraction was Na2CO3,1:20 of the ratio of sample to water,180 mins of extraction at 80℃, The rate was 6.82%.2.Choosing the optimal precipitator of methanol ethanol acetone, methanol would be the optimal precipitator, acetone is noxious but the ethanol is the cheapest, so we choose the ethanol; Sorite extraction method extrats fattiness; The separation of smaller molecules by 3000 Dalton dialysis membrane.3. De-color by activated carbon and hydrogen peroxide(H2O2). activated carbon is the best no side-effect and adsorbs some polysaccharide, polysaccharide would be oxidated by high consistence hydrogen peroxide. Sevag method was the optimal de-protein process.To sum up, the polysaccharide and the dissoluble protein was extracted and pured, and determined the molecular weight of the dissoluble protein.