Physicochemical Property And Extraction Studies Of The Polysaccharide And Chitin From Ganoderma Lucidum

Ganoderma lucidum is a kind of edible and medicinal fungi. Ganoderma lucidum polysaccharide with a variety of important pharmacological activities,such as anti-inflammatory,immunemodulatory, anti-tumor,liver,lowering blood sugar and blood pressure, and so on,have a good prospect of application.This article is about Ganoderma lucidum polysaccharide extraction process optimization,Ganoderma lucidum polysaccharide anti-oxidation, Ganoderma lucidum chitin/chitosan and the physical and chemical properties.The results are as follows:1.Ganoderma lucidum polysaccharide water extract of the optimization processSingle factor experiments,the best extraction conditions:ultrasonic time of 25 min;ultrasonic temperature 55℃;cellulase enzyme capacity 2000 u/g;neutral protease enzyme for the time 1.5 h,digestion temperature 40℃,for the amount of enzyme 1400 u/g,Under these conditions,the polysaccharide Ganoderma lucidum extract rate of 4.02 percent.2.Ganoderma lucidum polysaccharide alkali extract of the optimization processSingle-factor test,to be Ganoderma lucidum polysaccharide base to extract the best conditions are as follows:liquid ratio:1:50;extraction temperature:80℃;extraction time:6 h;the concentration of sodium hydroxide:1 mol/L.Extraction of a number of times is appropriate. Under these conditions,Ganoderma lucidum alkali extract polysaccharide can be achieve the extraction rate of 25.32%.3.Ganoderma lucidum polysaccharide anti-oxidationGanoderma lucidum extract from the above-mentioned three kinds of polysaccharide Ganoderma lucidum:Ganoderma lucidum to the water soluble polysaccharide,alkaline extraction of water soluble polysaccharide Ganoderma lucidum,alkaline extraction of alkali soluble polysaccharide Ganoderma lucidum were made three free radicals:DPPH·,·OH,ABTS~+'s Experimental anti-oxidation.The results show that three kinds of polysaccharide Ganoderma lucidum on the three have clear effects of free radicals,alkaline extraction of water soluble polysaccharide to remove the effects of free radicals better than the other two. 4.Ganoderma lucidum chitin/chitosan and the physical and chemical characteristicsBy the fruiting bodies of Ganoderma lucidum to extract polysaccharides and triterpenes after the residue,and the preparation of chitin chitosan,electron microscopy,infrared spectroscopy detection,GC analysis of Ganoderma lucidum chitin physical and chemical properties of the crab and shrimp shell the source of chitin,and through orthogonal experimental design preparation glucosamine hydrochloride.The innovation of this study:1.The use of ultrasound combined with the enzyme extracted polysaccharide,the water extract of Ganoderma lucidum polysaccharide extraction rate than the traditional method of extraction.2.The use of alkaline extract of Ganoderma lucidum with a high level and difficult to extract the polysaccharide,a greater degree of the lucidum polysaccharide to increase the extraction rate.3.By the fruiting bodies of Ganoderma lucidum to extract polysaccharides and triterpenes abandoned after the residue,has been prepared in the medical,pharmaceutical,chemical,food,cosmetics, printing and dyeing,paper making,agriculture,environmental protection, and enzyme immobilization,and other carriers have Extensive use of chitin,chitosan and glucosamine hydrochloride.