Study Of Technology For The Third Generation Of High Purity Fructose By Simulated Moving-bed Production

The fructose is the natural nutrition sweetening agent, the fructose has used in prescripition of functionality food, the nutrition health foods, in gradually cold drink food as well as low heat value food and the mode of motion drink in Europe and America, Japan and so on developed country. But our country active output was insufficient, and the F42 syrup hold dominant position,the fructose used in medicine market depends entirely on the import solution.This experiment had separated the third generation high-purity fructose through the simulation migration bed technology, the fructose purity above 99%.The purpose of our research was to define the key technology parameters of the preparation third generation high pure fructose. In this experiment, took the corn starch as raw materials, by using single factor experiment, multi factor combination experiment and sas data analysis software to research enzyme liquefication method,preparated glucose with saccharification,immobilization of glucose by magnetic chitosan,preparated frutose corn syrup with isomerization method,simulated moving bed technique in extraction of third-generation high-purity fructose and researched conditions of solvent extraction crystallization fructose, meanwhile, the ultraviolet spectra, infrared spectra, scanning electron microscope HPLC were applied to detect the characters of fructose.1. Research of Corn Starch Preparating Glucose. The effect on the yield ofα-amylase,liquefication time,the starch solution concentration,saccharification time and the glucoamylase were researched. Used single factor ctor experiment to define the changing range of all the factors, and used orthogonal combination experiment design to optimize the extraction technology parameters.The results were: theα-amylase was 15U/g starch,iquefication time was 30min,the starch solution concentration was 38%,the glucoamylase was 120U/g starch,saccharification time was 48h.The syrup flowed through ion exchange column two times,pH was 7.0, the DE value was 97.03%,color value was 0 (IU).So,the syrup after purification was colorless,and the syrup quality was nearly not affected.2.Reseach on the best technology conditions of immobilization glucose isomerase. The experiment fixed glucose isomerase with the magnetic compound microballoons method.⑴The size,morphology,structure,size distribution were studied by means of SEM,FT-IR,MASTERSIZE. The results indicate: the morphology of magnetic chitosan microspheres were all sphericity,distribution was narrow. FT-IR results show the structure of magnetic microspheres and the Fe3O4 particle was coated completely by the chitosan. It is a kind of superior carrier of immobilization of enzyme.⑵The optimized conditions were as following: the amount of enzyme 12mg/mL,glutaraldehyde5%, Cross-linking time 2h, oscillation time 6h. . There was the verification test with the best technological parameter, took the recovery of enzyme as the target, the result indicated the average recovery of enzyme was 84.8%.⑶The results of determined the nuture of the immobilized enzyme , the optimized conditions were as following: the Mg2+ concentration 0.01M, the Co2 + concentration 0.003M, the pH7.2, temperature 70℃.Through the experiment of kinetics of enzyme-catalyzed reaction, the imobilized enzyme in the catalyzed performance aspect, compared with the dissociation enzyme, the magnetic microballoons fossilization enzyme michaelis constant was big, the affinity changes was small, this possible concerned with the immobilized carrier shell polyose's spatial barrier and the proliferation limit influence3.Reseach of obtained isomerization of fructose syrup . This experimental studied using the enzyme column producted fructose syrup continuously. Used single factor ctor experiment, orthogonal experiment to study the influence of conversion rate with velocity syrup, syrup concentration , isomerase dosage.and so on three aspects.. The results were: syrup flow rate was 2.5mL/min, syrup concentration of 45%, the amount and sugar isomerase volume ratio was 0.8mL/mL. After the sugar refined heterogeneous , obtained the color value was 0 (IU), pH was 7.0 for fructose syrup, therefore, a colorless sugar after refining, in which fructose content of 43.80%.4. Reseach of the third generation of High Purity fructose by Simulated moving-bed production. In this study, the safety factor method was applied to simulated moving bed, to achieve the separation of fructose and glucose.⑴Through the single column pulse experiment, took the degree of dissociation as the target, carried on the single factor experiment, obtained Purolite PCR-642Ca2+ resin to have the good adsorption effect. Best sampling condition: column temperature was 60℃, 60% of sample concentration, elution flow rate was 1.62mL/min, injection volume was 18mL.⑵Used the single column pulse experiment obtained results, then through the safety factor method to be a simple parameter design method of SMB, determined that the SMB's theory parameters.⑶Simulated moving bed through the actual operation, the theoretical value will be optimized in the experiment, optimal preparation of high purity fructose SMB parameters: switching time 469s,elutriant flux 1.574L/h,feed flux0.325L/h,extraxt flux0.902L/h,raffinate flux 0.951L/h,recursion flux 1.897L/h.Under this operating condition, the third generation of high-purity fructose purity of 99.41%, yield of 80.32%. glucose purity of 92.34%,yield of 90.13 %.5. Research of crystallization fructose craft. This experiment used the solvent extraction crystallization fructose, took crystallizes the fructose rate as the target,used single factor ctor experiment, orthogonal experiment to study the influence of rate with liquid ratio, temperature, crystallization time and so on three factors . The results were: liquid ratio of 1:10, the temperature difference was 65-5℃, the crystallization time was 72h.