| Huai-ju was one of the four drugs, which were yam, Rehmannia, Achyranthes, chrysanthemum. Huai-Ju had the function Pinggan yang, commonly used in the treatment of liver embolism due to dizziness. Picking chrysanthemums at the same time, would produce a large number of stems, leaves, and yield more than larger than the chrysanthemum. Their stems and leaves contain a large number of flavonoids, such flavonoids have antioxidant, anti-aging treatment of cardiovascular disease, blood cholesterol and other health care function. In this article, we would choose Huai-Ju leaves as raw material, through the ultrasound-assisted method of extraction Huai-Ju leaves flavonoids and to study their restore ability.1 A single-factor test examined the use of liquid feed ratio, temperature, extraction time, particle size and other factors on pregnant chrysanthemum leaves flavonoids extraction rate of orthogonal experiment to be Jiaoyou extraction conditions were: liquid feed ratio is 20, the temperature was 60℃, extraction time of 30min, particle size 40 mesh. Under these conditions, pregnant with chrysanthemum leaves flavonoids extraction rate of 4.31%.2 200mL concentration of the Wye 11.9mg/mL thick chrysanthemum leaf flavonoid solution to 2mL/min the sample, 95% ethanol elution rate 0.8mL/min, collecting eluate, vacuum concentrated to 100mL, the measured concentration of 17.65mg/mL, the concentrated liquid low-temperature vacuum drying, was sallow Huai-Ju leaves flavonoids powder 2.32g, purity Pf of 76.22%.3 Huai-Ju leaves flavonoids remove hydroxyl radicals IC50 of 0.1173 g / L. 1.5mg/mL flavonoids solution to restore the force of 1.35 absorbance; adding low-temperature intestine can be significantly reduced during the low-temperature storage intestines CD, TBARS and carbonyl groups increased.
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