| Cordyceps militaris is substitute of valuable medicinal and edible fungi of Cordyceps sinensis which contains polysaccharide, protein, amino acid and other active ingredients. This paper studied the influence of artificial culture conditions and culture medium for growth and active ingredients of Cordyceps militaris in order to provide a certain basic data for the high quality and yield of artificial cultivation technology of Cordyceps militaris.1. Studied the influence of culture conditions（culture substrate, medium usage, ratio of material to solution, inoculation quantity, and initial pH of medium, culture time） on growth of Cordyceps militaris. Through each single factor and orthogonal experiments to obtain the best artificial culture conditions of Cordyceps militaris, of which medium usage was 40 g, ratio of material to solution was 1:1.4, inoculation quantity was 3.2 mL and culture time was 60 d.2. Studied the influence of culture medium（carbon source concentration, nitrogen source concentration, mineral element concentration） on growth and active ingredients of Cordyceps militaris and determined the best concentration by mass concentration gradient method. Through each single factor and orthogonal experiments to obtain the best culture medium of Cordyceps militaris, of which glucose concentration was 20 g·L-1, yeast powder concentration was 10 g·L-1, dihydrogen phosphate concentration was 3 g·L-1 and magnesium sulphate concentration was 2 g·L-1, stroma dry weight was 6.11 g, polysaccharide content was 3.49 g·100g-1 in optimized medium. The stroma dry weight and polysaccharide content in optimized medium were significantly higher than original medium.
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