| Cordyceps militaris is one of the important traditional Chinese medicine (TCM), also the common substitutes of expensive TCM Cordyceps sinensis. Cordyceps militaris contains polysaccharides, cordycepin, nucleoside substances, ergosterol, cordyceps acid, and other active ingredients, these effective ingredients are important indicator of the quality of Cordyceps militaris fruiting bodies. The artificial cultivation Cordyceps militaris including liquid fermentation and solid-state fermentation, research on the effective constituents of the Cordyceps militaris mainly in liquid fermentation, while the solid state fermentation mostly focus on the optimization growth conditions of the fruitbody. The paper studies on the effect of cultivating conditions and medium to the gorwth of fruitbody Cordyceps militaris and its effective components, providing certain basis of synthesis of high quality Cordyceps militaris and the main active material production base.Studied the culture conditions (light, temperature, cultivating time) on the primordium formation and fruitbody growth and effective ingredient content, the main results are as follows:(1) Illumination is one of the most impotant factors of Cordyceps militaris fruitbody growth and effective component synthesising. The stronger light, polysaccharide, adenosine, carotenoids content was higher, the weaker light is more conducive to yield increase and cordycepin, ergosterol, soluble protein and free amino acid synthesis. Extending the time of light will boost the growth of carotenoids, polysaccharide, adenosine and cordyceps acid synthesis, but restrain the fruitbody growth and soluble protein synthesising. For synthesis of polysaccharide, cordycepin and carotenoids, the incandescent light is much better than yellow and red light, superior to ergosterol and free-amino acids oppositely. Comprehensive consideration, determine the appropriate illumination conditions for:the incandescence light of 200 lx, the primordium forming stage for 24 h/d, while 16 h/d for fruitbody growth stage is the best.(2) Different temperature (20℃,24℃,22℃and 26℃) have different effect to the fruitbody yield and effective ingredients synthesis. When the temperature increases from 20℃to 26℃, the primordium can not be properly formed. From 20-24℃, the fruiting body and cordyceps acid of production decreased significantly as the temperature increasing; for the content of polysaccharides, cordycepin, it has significantly difference between those three culture temperatures, while,22℃for the best; 20℃and 22℃, culture temperature, the bodies of adenosine, carotenoid content are significantly higher than that of 24℃, soluble protein is opposite; for the synthesis of amino acids,22℃and 20℃was significantly higher than 24℃. Comprehensive analysis of fruiting body yield and composition, to determine Cordyceps militaris cultured strict temperature range 20-22℃.(3) The production and effective ingredient content varied by the cultivation time. The fruitbody after inoculation 40d to maximum yield, besides polysaccharides, other effective ingredients are accumulated greatly at the esse bloom period, but the cordcepin increased lager as prolonger the culrivation time. Except for cordycepin, soluble protein and amino acid, the other 5 elements reached their highest level then turned down as the extension of time, after more than 40d the fruit color went black. Considing the production and color, content of cordycepin and other ingredients content, determined the optimal harvest time should choose the maximum yield of fruitbody after 5 d, that's to say, about 45 days after inoculation cultivation.2 Researched the effect of substrate (carbon sources, nitrogen source, inorganic salt, vitamin, pH) on the growth of fruitbody and effective ingredient content, the main results are as follows:(1) For the fruitbody growth, maltose and mannitol is the best. Different carbon source have vary influence on different availability composition synthesis,. Analyse and contrast the effect of maltose and mannitol on cordycepin adenosine cordyceps acid and various effective component synthesis and accumulation, determine the most appropriate carbon source is mannitol.(2) All sorts of nitrogen additive have different effect to the grow and effective ingredient of Cordyceps militaris synthesis. Considing the fruitbody production, free ergostrerol, cordycepin and soluble protein content, organic nitrogen source will significantly better than inorganic nitrogen source, while the cordycep acid is opposite. Organic nitrogen source of fresh pupa can promote 5 kind of effective components's synthesis (polysaccharide, cordycepin, cordycep acid, soluble protein and carotenoids), inorganic nitrogen source of synthesis and diammonium phosphate have obvious role in promoting the accumulation of 4 kinds of effective ingredients (cordyceps acid, adenosine, free amino acid and carotenoids). So the best source of organic nitrogen additive is fresh pupa, and inorganic nitrogen source is diammonium phosphate.(3) Vitamins as the trace growth factor for Cordyceps militaris growth has obvious effect. VB1 can promote 3 kinds of the effective components (cordycep acid, free amino acid and carotenoids), also promote the fruitbody growth, finally VB1 chosen as the best additive.(4) Of different inorganic salts on the cumulative effects of different active ingredients in different. Through contrast KH2PO4 and NaNO3, which can promote most types of effective components, by the effect of polysaccharide, cordycepin, adenosine of effective ingredients of synthesis and fruitbody, finally the former chosen as the better additive.(5) Medium pH value has various different effects on the synthesis of the active ingredient. For the synthesis of polysaccharide, coedycepin, cordyceps acid, free ergosterol, free amino acid and carotenoids acidic conditions, acidity is more advantageous; On the neutral condition, soluble protein content is the highest. Comprehensive consideration, medium pH Cordyceps militaris should be adjustable betweent 5.5-6.5.
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