Effects And Mechanisms Of The Traditional Chinese Medicine On Improving Cold-stress-resistance

Seventy-two male Wistar rats at 90d of age weight range from 190 to 210g were randomly divided into six groups and three traditional medicine formulas for screening were all composed of several component that have the function of enhance the ability of cold-stress-resisitance,the positive control formula was composed of vitamin C,vitamin E and vitamin B2.The groups 1 ,group2 and group3 were treated with three traditional medicine formula 1,2 and 3 ,the group 4 was treated with vitamins formula as positive control group and group 5 and group 6 were treated with water as negative control group and blank control group respectively.A1l the groups were administrated for 2 weeks and the cold-stress-resistance effect of the traditional medicine formulas and the vitamin fonnula to rats were examined through cold water swimming water (10 0C) and serum hormones concentration including adrenoeorticotropic hormone (ACTH),glucogon,free T4 ,cortisol concertation related to cold-stressresistance were detected and adrenal gland zona fasiculata cell ultra microstructure were observed.The results showed that the swimming time of three traditional Chinese medicine formulas group rats were significantly longer (NO.01) than that of negative control group rats, and longer than the positive control group (P<0.05),and the cold swimming time of formula 1 was longer than the group2,group 3 and positive control group.The serum cortisol concentration of the Chinese traditional medicine formula group rats and positive control group rats were higher significantly than that of negative control group. The serum ACTH concentration of traditional Chinese medicine formulas group rats and positive control rats didn抰 have difference with that of negative control group, but the serum glucagon concentration of traditional Chinese medicine formulas group rats and positive group rats were higher significantly than that of negative control group. The33serum free T4 concentration of positive control group were higher significantly than that of negative control group, but that of traditional Chinese medicine formulas group were lower than that of negative control group with insignificant difference,the mitochondria damage of negative control group was serious than the traditional medicine formula groups and the positive group. It was concluded that the vitamins formula can enhance the cold-stress-resistance of rats through alter the serum concentration and lessen the impairment to cell membrane and organelle caused by superoxide and accelerating energy metabolism, but three traditional Chinese medicinal formulas agents could improve the cold-stress-resistance of rats through complicated mechanism including regulate the activity of neuroendocrine and lighten the cold stress respons.