Study On Microorganism Of Rumex Patientia Rhizosphere Soil

Rumex patientia (R. patientia) is the second largest genus Polygonum genussorrel, there are about150species. R. patientia is rich in our country, with26species,distributed in provinces and regions. R. patientia contained in the "Shen Nong’sHerbal Classic". The herbal medicine in our country folk be used to stop bleeding andtreat scabies. Modern pharmacological studies have shown that it has anti-fungal,anti-tumor, anti-viral and anti-oxidative effects. Chemical analysis showed it has arich anthraquinone, tannic acid, naphthalene derivatives. In this thesis, Firstly, Wediscussed R. patientia rhizosphere and non-rhizosphere soils microbial diversity inJilin City four habitats using microbial culture and non-culture methods; secondly, wediscussed R. patientia rhizosphere and non-rhizosphere soils microorganisms inChangchun City two habitats using microbial culture method; Finally, we analyzedsoil microorganisms of R. patientia of similar habitats in Changchun and Jilin City.The main results are listed as following:1. This study suggested that the rhizosphere microorganisms mainly becomposed of bacteria, actinomycetes, followed by fungi least. The microbialpopulation is more in rhizosphere soils compared to non-rhizosphere soils of R.patientia of four habitats in Jilin City.(1) The principal component analysis (PCA) biplot displayed that azotobacterand ammonifier was the strongest determinant for rhizosphere soils, and total bacteriaand actinomycetes were also the important factor for rhizosphere soils; Whereassulfur bacteria, total bacteria and ammonifier was the strongest determinant fornon-rhizosphere soils and actinomycetes and fungi were also the important factor fornon-rhizosphere soils.(2) Pearson correlation coefficient analysis showed R. patientia rhizosphere ofJilin four habitats actinomycetes richness was significantly negatively correlated withmoisture content and humic matter, the abundance of ammonifier was significantlynegatively correlated with total P, azotobacter richness was very significant negativecorrelated with total P. In the non-rhizosphere soil, the total bacteria richness wassignificantly negatively correlated with total K, actinomycetes richness wassignificantly positively correlated with pH, and azotobacter richness weresignificantly negatively correlated with pH.2. This study suggested that the microbial population is more in rhizospherecompared to non-rhizosphere soils of R. patientia of two habitats in Changchun City. 3. We compared rhizosphere soil microorganisms of R. patientia of similarhabitats between Jilin and Changchun City, These results show that the numbers ofrhizosphere soil microorganisms of R. patientia at habitats near water and sunnyhabitats in Jilin City were higher than Changchun City.4. Our results indicate that unique clones in rhizosphere and non-rhizospheresoils are more than common clones in both of them in4different habitats in Jilin by16SrDNA technology, Furthermore, the unique clones in rhizosphere soil are morethan that in non-rhizosphere soil. The study results show that rhizosphere microbialdiversity is rich in R. patientia of rhizosphere soil and it has more number ofcommunities in rhizosphere soil, it has soil improvement effect.